NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, California, USA.
Compr Physiol. 2013 Apr;3(2):941-56. doi: 10.1002/cphy.c120025.
We review the biomedical applications of Raman spectroscopy at the single cell and tissue level. Raman scattering is the inelastic scattering of light by molecular bonds resulting in a wealth of spectral bands, which enable the identification of biological materials and the nondestructive analysis of dynamic changes in their biochemistry. We briefly review the basics behind highly sensitive Raman spectroscopy and highlight recent applications to biomedical research. We discuss advanced chemometrics methods that are utilized to analyze Raman spectral data and which permit one, for example, to distinguish between normal and diseased cells or which enable one to follow the differentiation of stem cells without perturbing the cellular biochemistry. We also discuss advanced coherent Raman scattering techniques, such as coherent anti-Stokes Raman scattering and stimulated Raman scattering, which allow for the molecularly specific imaging of cells, tissues, and entire organisms in vitro and in vivo.
我们回顾了在单细胞和组织水平上拉曼光谱的生物医学应用。拉曼散射是分子键的非弹性光散射，产生丰富的光谱带，从而能够识别生物材料，并对其生物化学的动态变化进行非破坏性分析。我们简要回顾了高灵敏度拉曼光谱背后的基础知识，并重点介绍了最近在生物医学研究中的应用。我们讨论了用于分析拉曼光谱数据的高级化学计量学方法，这些方法可以帮助区分正常细胞和病变细胞，或者在不干扰细胞生物化学的情况下跟踪干细胞的分化。我们还讨论了先进的相干拉曼散射技术，如相干反斯托克斯拉曼散射和受激拉曼散射，这些技术可以在体外和体内对细胞、组织和整个生物体进行分子特异性成像。
