Moura Catarina Costa, Tare Rahul S, Oreffo Richard O C, Mahajan Sumeet
Department of Chemistry and Institute for Life Sciences, University of Southampton, Highfield Campus, Southampton SO17 1BJ, UK Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, UK.
Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, UK.
J R Soc Interface. 2016 May;13(118). doi: 10.1098/rsif.2016.0182.
The use of skeletal stem cells (SSCs) for cell-based therapies is currently one of the most promising areas for skeletal disease treatment and skeletal tissue repair. The ability for controlled modification of SSCs could provide significant therapeutic potential in regenerative medicine, with the prospect to permanently repopulate a host with stem cells and their progeny. Currently, SSC differentiation into the stromal lineages of bone, fat and cartilage is assessed using different approaches that typically require cell fixation or lysis, which are invasive or even destructive. Raman spectroscopy and coherent anti-Stokes Raman scattering (CARS) microscopy present an exciting alternative for studying biological systems in their natural state, without any perturbation. Here we review the applications of Raman spectroscopy and CARS imaging in stem-cell research, and discuss the potential of these two techniques for evaluating SSCs, skeletal tissues and skeletal regeneration as an exemplar.
将骨骼干细胞（SSCs）用于基于细胞的治疗目前是骨骼疾病治疗和骨骼组织修复最具前景的领域之一。对SSCs进行可控修饰的能力可为再生医学提供巨大的治疗潜力，有望使宿主被干细胞及其后代永久性重新填充。目前，评估SSCs向骨、脂肪和软骨的基质谱系分化采用的不同方法通常需要细胞固定或裂解，这些方法具有侵入性甚至破坏性。拉曼光谱和相干反斯托克斯拉曼散射（CARS）显微镜为在自然状态下研究生物系统提供了一种令人兴奋的替代方法，不会产生任何干扰。在此，我们综述拉曼光谱和CARS成像在干细胞研究中的应用，并以评估SSCs、骨骼组织和骨骼再生为例讨论这两种技术的潜力。
