Department of Chemistry, University of Science and Technology of China, Anhui, PR China.
J Sep Sci. 2013 Aug;36(15):2486-95. doi: 10.1002/jssc.201300294. Epub 2013 Jul 3.
Sucrose esters (SEs) were successfully extracted from Oriental tobacco leaves using a new methodology based on accelerated solvent extraction followed by hydrophilic-lipophilic balanced cartridge cleanup step. The SEs were detected by HPLC with ion-trap MS detection using an electrospray interface operated in the positive ion mode. This methodology combines the high efficiency of extraction provided by a pressurized fluid and the highly sensitive characterization offered by ion-trap MS. Under the optimized conditions, 14 SEs were first identified among a total of 23 SEs found in Oriental tobacco leaves. Under the same conditions, only four new SEs were extracted by using traditional ultrasound-assisted extraction and liquid-solid extraction methods. The present method might be potentially useful in high-efficiency extraction and sensitive characterization of SEs from complex matrices such as tobacco leaves.
采用基于加速溶剂萃取和亲水-亲脂平衡柱净化步骤的新方法，成功地从东方烟草叶片中提取了蔗糖酯（SEs）。采用高效液相色谱-离子阱质谱联用技术（HPLC-IT-MS），以电喷雾接口正离子模式进行检测，对 SEs 进行了检测。该方法结合了加压流体萃取的高效率和离子阱 MS 提供的高灵敏度的特点。在优化条件下，从东方烟草叶片中共鉴定出 23 种 SEs 中的 14 种。在相同条件下，仅采用传统的超声辅助提取和液-固萃取方法，可提取 4 种新的 SEs。本方法可能在高效提取和对复杂基质（如烟草叶片）中的 SEs 进行敏感的特征描述方面具有潜在的应用价值。
