Simonovska Breda, Srbinoska Marija, Vovk Irena
National Institute of Chemistry, Laboratory for Food Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
J Chromatogr A. 2006 Sep 15;1127(1-2):273-7. doi: 10.1016/j.chroma.2006.05.103. Epub 2006 Jul 3.
Sucrose esters from the surface of leaves of Nicotiana tabacum L. have been shown to possess interesting biological activities. We developed a simple and effective method for their analysis using HPTLC silica gel plates, n-hexane-ethyl acetate (1:3, v/v) as developing solvent and aniline-diphenylamine as a detection reagent. Off-line TLC-MS was also used for the detection and identification of the compounds. Solutions containing sucrose esters upon alkaline hydrolysis give sucrose, which is used for indirect estimation by TLC of the sucrose ester content. The method is applicable for the screening for sucrose esters in plant extracts. The extract obtained from the surface of green leaves of oriental tobacco type Prilep P-23 contains sucrose esters and is effective against Myzus persicae (Sulzer) in laboratory and field experiments.
烟草叶片表面的蔗糖酯已被证明具有有趣的生物活性。我们开发了一种简单有效的分析方法，使用高效薄层色谱硅胶板，正己烷 - 乙酸乙酯（1:3，v/v）作为展开溶剂，苯胺 - 二苯胺作为检测试剂。离线薄层色谱 - 质谱联用也用于化合物的检测和鉴定。含有蔗糖酯的溶液在碱性水解后产生蔗糖，通过薄层色谱法间接估算蔗糖酯含量。该方法适用于植物提取物中蔗糖酯的筛选。从东方烟草品种普里莱普P - 23的绿叶表面获得的提取物含有蔗糖酯，在实验室和田间试验中对桃蚜（苏尔泽）有效。
