School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
Analyst. 2013 Aug 7;138(15):4340-4. doi: 10.1039/c3an00500c. Epub 2013 May 30.
Suspensions of electrocatalytic platinum nanoparticles with radii as small as 78.9 ± 3.5 nm that are functionalised with DNA only in one region have been created using templated electrodeposition. The integrity of the bound DNA following nanoparticle desorption from the electrode is demonstrated by detecting attomolar concentrations of DNA without the need for molecular, e.g., PCR or NASBA, amplification. Double potential step approaches coupled with interface engineering via nucleation sites allows PtNPs to be created with controlled particle size and density in a facile and reproducible manner.
使用模板电沉积技术制备了具有仅在一个区域功能化的 DNA 的电催化铂纳米粒子悬浮液，其半径小至 78.9 ± 3.5nm。通过检测无需分子（例如 PCR 或 NASBA）扩增的纳摩尔浓度的 DNA，证明了纳米粒子从电极上解吸后结合的 DNA 的完整性。通过双电位阶跃方法和通过成核位点的界面工程，以简单且可重复的方式可以制备具有受控粒径和密度的 PtNPs。
