School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
Chem Commun (Camb). 2012 Jan 21;48(6):838-40. doi: 10.1039/c1cc15115k. Epub 2011 Dec 2.
Suspensions of electrocatalytic gold nanoparticles with radii as small as 83 ± 13 nm that are functionalised with DNA only in one region have been created using templated electrodeposition. The integrity of the bound DNA following nanoparticle desorption from the electrode is demonstrated by detecting picomolar concentrations of DNA without the need for molecular, e.g., PCR or NASBA, amplification.
使用模板辅助电化学沉积的方法制备了具有仅在一个区域功能化 DNA 的电催化金纳米粒子的悬浮液，其半径小至 83 ± 13nm。通过检测皮摩尔浓度的 DNA 而无需分子扩增（例如 PCR 或 NASBA），证明了从电极上解吸纳米粒子后结合的 DNA 的完整性。
