使用 Vitek 2 抗菌药敏分析系统鉴定耐甲氧西林金黄色葡萄球菌中的 mecC。
Use of Vitek 2 antimicrobial susceptibility profile to identify mecC in methicillin-resistant Staphylococcus aureus.
机构信息
Department of Medicine, University of Cambridge, Cambridge, United Kingdom.
出版信息
J Clin Microbiol. 2013 Aug;51(8):2732-4. doi: 10.1128/JCM.00847-13. Epub 2013 May 29.
Abstract
The emergence of mecC methicillin-resistant Staphylococcus aureus (MRSA) poses a diagnostic challenge for clinical microbiology laboratories. Using the Vitek 2 system, we tested a panel of 896 Staphylococcus aureus isolates and found that an oxacillin-sensitive/cefoxitin-resistant profile had a sensitivity of 88.7% and a specificity of 99.5% for the identification of mecC MRSA isolates. The presence of the mecC gene, determined by bacterial whole-genome sequencing, was used as the gold standard. This profile could provide a zero-cost screening method for identification of mecC-positive MRSA strains.
摘要
耐甲氧西林金黄色葡萄球菌（MRSA）中 mecC 基因的出现给临床微生物学实验室的诊断带来了挑战。我们使用 Vitek 2 系统对 896 株金黄色葡萄球菌进行了检测，发现对 mecC 型耐甲氧西林金黄色葡萄球菌的检测，其 oxacillin 敏感/cefoxitin 耐药表型的敏感性为 88.7%，特异性为 99.5%。以细菌全基因组测序来确定 mecC 基因的存在作为金标准。这种表型可以提供一种零成本的筛查方法，用于鉴定 mecC 阳性的耐甲氧西林金黄色葡萄球菌菌株。
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