Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK.
J Antimicrob Chemother. 2012 Dec;67(12):2809-13. doi: 10.1093/jac/dks329. Epub 2012 Aug 31.
A previously unidentified mecA homologue, mecA(LGA251), has recently been described in methicillin-resistant Staphylococcus aureus (MRSA) from humans and dairy cattle. The origin and epidemiology of this novel homologue are unclear. The objective of this study was to provide basic descriptive information of MRSA isolates harbouring mecA(LGA251) from a range of host animal species.
A number of S. aureus isolates from historical animal isolate collections were chosen for investigation based on their similarity to known mecA(LGA251) MRSA isolates. The presence of mecA(LGA251) was determined using a multiplex PCR and antimicrobial susceptibility testing performed by disc diffusion.
MRSA harbouring mecA(LGA251) were found in isolates from a domestic dog, brown rats, a rabbit, a common seal, sheep and a chaffinch. All of the isolates were phenotypically MRSA, although this depended on which test was used; some isolates would be considered susceptible with certain assays. All isolates were susceptible to linezolid, rifampicin, kanamycin, norfloxacin, erythromycin, clindamycin, fusidic acid, tetracycline, trimethoprim/sulfamethoxazole and mupirocin. Five multilocus sequence types were represented (2273, 130, 425, 1764 and 1245) and six spa types (t208, t6293, t742, t6594, t7914 and t843).
The discovery of MRSA isolates possessing mecA(LGA251) from a diverse range of host species, including different taxonomic classes, has important implications for the diagnosis of MRSA in these species and our understanding of the epidemiology of this novel mecA homologue.
最近在耐甲氧西林金黄色葡萄球菌（MRSA）中发现了一种以前未被识别的 mecA 同源物，mecA（LGA251）。这种新型同源物的起源和流行病学尚不清楚。本研究的目的是为来自各种宿主动物物种的携带 mecA（LGA251）的 MRSA 分离株提供基本描述信息。
根据与已知 mecA（LGA251）MRSA 分离株的相似性，选择历史动物分离物收集的一些金黄色葡萄球菌分离物进行调查。使用多重 PCR 确定 mecA（LGA251）的存在，并通过圆盘扩散进行抗生素敏感性测试。
在一只家犬、褐鼠、一只兔子、一只普通海豹、绵羊和一只雀形目鸟的分离物中发现了携带 mecA（LGA251）的 MRSA。所有分离物均为表型 MRSA，尽管这取决于使用哪种测试；某些分离物在某些检测中可能被认为是敏感的。所有分离物均对利奈唑胺、利福平、卡那霉素、诺氟沙星、红霉素、克林霉素、夫西地酸、四环素、复方磺胺甲噁唑和莫匹罗星敏感。代表了五种多位点序列类型（2273、130、425、1764 和 1245）和六种 spa 型（t208、t6293、t742、t6594、t7914 和 t843）。
从包括不同分类群在内的多种宿主物种中发现携带 mecA（LGA251）的 MRSA 分离株，这对这些物种中 MRSA 的诊断以及我们对这种新型 mecA 同源物的流行病学的理解具有重要意义。
