Wang Wei, Li Tan, Han Guang, Li Ying, Shi Li-Hua, Li Hui
Department of Urology, The Affiliated Hospital of Logistics University of the Chinese People's Armed Tianjin, 300162, China.
Indian J Biochem Biophys. 2013 Apr;50(2):87-92.
To investigate the biological function of microRNA-34a (miR-34a) in bladder cancer, the expression of miR-34a was determined using quantitative real-time polymerase chain (qRT-PCR) reaction in 42 cases of bladder cancer. The relationship between the expression of miR-34a and development of bladder cancer was also studied. The mature mimics of miR-34a were chemically synthesized and transiently transfected into human bladder cancer T24 cells. The effects of miR-34a on apoptosis, cell cycle and proliferation in T24 cells were evaluated by flow cytometry and MTT, respectively. The results showed that the low expression rate of miR-34a was correlated with the malignancy and tumor size of bladder cancer. The up-regulation of miR-34a in T24 cells contributed to cell growth and cell cycle arrest, but not caspase-3 pathway. These findings suggest that the relative low expression of miRNA-34a might be involved in the tumorigenesis of bladder cancer.
为了研究微小RNA-34a（miR-34a）在膀胱癌中的生物学功能，采用定量实时聚合酶链反应（qRT-PCR）检测了42例膀胱癌组织中miR-34a的表达情况，并研究了miR-34a表达与膀胱癌发生发展的关系。化学合成miR-34a的成熟模拟物并将其瞬时转染到人膀胱癌T24细胞中，分别通过流式细胞术和MTT法评估miR-34a对T24细胞凋亡、细胞周期和增殖的影响。结果显示，miR-34a低表达率与膀胱癌的恶性程度和肿瘤大小相关。T24细胞中miR-34a的上调促进了细胞生长和细胞周期阻滞，但不涉及半胱天冬酶-3途径。这些发现提示，miRNA-34a相对低表达可能参与了膀胱癌的肿瘤发生过程。
