[过渡金属化合物对还原硫酸盐的醋酸脱硫单胞菌超氧化物歧化酶活性的影响]
[Influence of transition metal compounds on superoxide dismutase activity of sulfur reducing Desulfuromonas acetoxidans bacteria].
作者信息
Vasyliv O M, Hnatush S O
出版信息
Mikrobiol Z. 2013 Mar-Apr;75(2):37-44.
Superoxide dismutase, as one of the enzymes of cells' antioxidant defensive system, catalyzes superoxide anion-radical (O2-) dismutation with O2 and H2O2 forming. The influence of such transition metal compounds, as FeSO4, FeCl3, MnCl2, NiCl2, and CoCl2 on superoxide dismutase activity of sulfur-reducing Desulfuromonas acetoxidans bacteria has been investigated. Maximal activity of the investigated enzyme has been observed accordingly under the influence of 1.0 mM of NiCl2, 2.0 mM of CoCl2 and MnCl2 on the second day and under the influence of 1.0 mM of FeCl3 and FeSO4 respectively, on the third day of growth in comparison with control samples. An increase of incubation time and concentration of metal compound in the medium caused the inhibition of superoxide dismutase activity.
超氧化物歧化酶作为细胞抗氧化防御系统的酶之一，催化超氧阴离子自由基（O2-）歧化生成O2和H2O2。研究了FeSO4、FeCl3、MnCl2、NiCl2和CoCl2等过渡金属化合物对还原型脱硫单胞菌超氧化物歧化酶活性的影响。与对照样品相比，在生长的第二天，分别在1.0 mM NiCl2、2.0 mM CoCl2和MnCl2的影响下观察到所研究酶的最大活性，在生长的第三天，分别在1.0 mM FeCl3和FeSO4的影响下观察到最大活性。培养基中孵育时间和金属化合物浓度的增加导致超氧化物歧化酶活性受到抑制。
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