Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg, Deutschhausstrasse 17a, 35037 Marburg, Germany, and Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany.
Org Lett. 2013 Jun 21;15(12):3062-5. doi: 10.1021/ol401247s. Epub 2013 May 30.
The behavior of five cyclic dipeptide prenyltransferases, responsible for C2-regular, C2-reverse, or C3-reverse prenylation, was investigated in the presence of the unnatural alkyl donors monomethylallyl and 2-pentenyl diphosphate. Both substrates were well accepted by the tested enzymes. Interestingly, C2-reverse and C3-reverse monoalkylated derivatives were identified as enzyme products in all of the enzyme assays. These findings indicate their similar reaction characteristics in the presence of unnatural alkyl donors.
五种环二肽类异戊烯基转移酶的行为，负责 C2-规则、C2-反向或 C3-反向异戊烯基化，在非天然烷基供体单甲基丙烯基和 2-戊烯基二磷酸的存在下进行了研究。两种底物都被测试的酶很好地接受了。有趣的是，在所有的酶促反应中，C2-反向和 C3-反向单烷基化衍生物被鉴定为酶产物。这些发现表明，在非天然烷基供体存在的情况下，它们具有相似的反应特性。
