Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
J Nat Med. 2020 Jun;74(3):501-512. doi: 10.1007/s11418-020-01393-x. Epub 2020 Mar 17.
Aromatic prenyltransferases (PTases), including ABBA-type and dimethylallyl tryptophan synthase (DMATS)-type enzymes from bacteria and fungi, play important role for diversification of the natural products and improvement of the biological activities. For a decade, the characterization of enzymes and enzymatic synthesis of prenylated compounds by using ABBA-type and DMATS-type PTases have been demonstrated. Here, I introduce several examples of the studies on chemoenzymatic synthesis of unnatural prenylated compounds and the enzyme engineering of ABBA-type and DMATS-type PTases.
芳基 prenyltransferases (PTases)，包括细菌和真菌中的 ABBA 型和二甲基烯丙基色氨酸合酶 (DMATS) 型酶，对天然产物的多样化和生物活性的提高起着重要作用。十年来，已经证明了使用 ABBA 型和 DMATS 型 PTases 对酶的特性和prenylated 化合物的酶促合成进行了研究。在这里，我介绍了一些关于非天然 prenylated 化合物的化学酶合成和 ABBA 型和 DMATS 型 PTases 的酶工程的研究实例。
