Research Institute for Chromatography, President Kennedypark 26, B-8500 Kortrijk, Belgium.
J Chromatogr A. 2013 Nov 22;1317:230-8. doi: 10.1016/j.chroma.2013.04.033. Epub 2013 Apr 30.
Gas chromatography coupled to isotope ratio mass spectrometry after on-line combustion (GC-C-IRMS) and high temperature conversion (GC-HTC-IRMS) is used for compound specific isotope ratio determination. This determination can only be performed successfully if the target solutes are fully resolved from other compounds. A new instrumental set-up consisting of heart-cutting two-dimensional GC based on capillary flow technology and a low thermal mass GC oven in combination with an isotope ratio mass spectrometer is presented. Capillary flow technology was also used in all column and interface connections for robust and leak-free operation. The new configuration was applied to the characterization of wax compounds in tobacco leaf and corresponding smoke samples. It is demonstrated that high accuracy is obtained, both in the determination of δ(13)C and δ(2)H values, allowing the study of biosynthesis and delivery mechanisms of naturally occurring compounds in tobacco.
气相色谱-同位素比质谱联用（GC-C-IRMS）和在线燃烧（GC-C-IRMS）及高温转化（GC-HTC-IRMS）用于化合物的特定同位素比测定。如果目标溶质能与其他化合物完全分离，则可成功进行该测定。本文介绍了一种新的仪器设备，它由基于毛细管流技术的二维中心切割气相色谱和低热质量气相色谱炉与同位素质谱仪组成。在所有柱和接口连接中都使用毛细管流技术，以实现可靠且无泄漏的操作。该新型配置应用于烟草叶和相应的烟雾样品中蜡质化合物的特征描述。结果表明，在测定 δ(13)C 和 δ(2)H 值时，均能获得高精度，这使得能够研究烟草中天然化合物的生物合成和传递机制。
