Cambridge Institute for Medical Research, Wellcome Trust/MRC Building, Addenbrooke's Hospital, Cambridge CB2 0XY, UK.
Trends Cell Biol. 2013 Nov;23(11):522-8. doi: 10.1016/j.tcb.2013.04.010. Epub 2013 May 28.
Endosomal protein sorting governs the fate of many physiologically important proteins involved in a panoply of cellular functions. Recent discoveries have revealed a vital role for endosomally localised branched actin patches in facilitating protein sorting. The formation of the actin patches has been shown to require the function of the WASH complex - the major endosomal actin polymerisation-promoting complex - which stimulates the activity of the ubiquitously expressed Arp2/3 complex. Another key component of the endosomal protein-sorting machinery is the retromer complex. Studies now show that retromer mediates the recruitment of the WASH complex and its regulators to endosomes. In this review, recent progress in understanding the role of the WASH complex along with retromer in endosomal protein sorting is discussed.
内体蛋白分选控制着许多生理上重要的蛋白质的命运，这些蛋白质参与了多种细胞功能。最近的发现揭示了内体定位的分支肌动蛋白斑在促进蛋白质分选中的重要作用。肌动蛋白斑的形成需要 WASH 复合物的功能——WASH 复合物是主要的内体肌动蛋白聚合促进复合物——它刺激普遍表达的 Arp2/3 复合物的活性。内体蛋白分选机制的另一个关键组成部分是逆行转运复合物。研究表明，逆行转运复合物介导 WASH 复合物及其调节剂到内体的募集。在这篇综述中，讨论了最近在理解 WASH 复合物以及逆行转运复合物在内体蛋白分选中的作用方面的进展。
