Boukhari N, Taleb-Senouci D, Chabane F Z, Besbes M, Lamri-Senhadji M Y
Laboratoire de nutrition clinique et métabolique (LNCM), faculté des sciences, département de biologie, université d'Oran, BP 1524, El M'Nouer, 31100 Oran, Algérie.
Ann Cardiol Angeiol (Paris). 2013 Jun;62(3):149-54. doi: 10.1016/j.ancard.2013.04.007. Epub 2013 Apr 19.
To valorize fish by-products oil by investigating its effects on dyslipidemia, hyperglycemia, reverse cholesterol transport and paraoxonase-1 activity in obese rat.
Sixteen male Wistar rats were fed a high fat diet. At 400 ± 10 g, obese rats were randomly divided into two groups: the first received 20% of sardine by-products oil and the second 20% of the edible portion oil. At d28, glycemia and serum lipids concentrations were estimated. High density lipoproteins (HDL2 and HDL3) were separated and their contents and composition in lipids and apolipoproteins were analyzed. Lecithin: cholesterol acyltransferase and paraoxonase-1 activities were assessed.
In group which consumed sardine by-products oil, serum cholesterol and triacylglycerols were reduced (-8% and -36%, respectively). However, glycemia was similar. HDL3-phospholipids, HDL3-unesterified cholesterol and HDL3-apolipoproteins were decreased by 56%, 10% and 12%, respectively. Lecithin: cholesterol acyltransferase activity was increased by 35% and the content of HDL2-cholesteryl esters was elevated by 12%. Serum paraoxonase-1 activity was increased by 25%.
In obese rat, sardine by-products oil may have a protective effect against cardiovascular risk by improving the anti-atherogenic metabolic pathway of cholesterol and triacylglycerols. This anti-atherogenic action is particularly enhanced by the increase in paraoxonase-1 activity which protects lipoproteins from oxidation.
通过研究鱼副产品油对肥胖大鼠血脂异常、高血糖、胆固醇逆向转运和对氧磷酶-1活性的影响，评估其价值。
16只雄性Wistar大鼠喂食高脂饮食。体重达到400±10克时，肥胖大鼠随机分为两组：第一组给予20%的沙丁鱼副产品油，第二组给予20%的可食用部分油。在第28天，测定血糖和血脂浓度。分离高密度脂蛋白（HDL2和HDL3），分析其脂质和载脂蛋白的含量及组成。评估卵磷脂胆固醇酰基转移酶和对氧磷酶-1活性。
食用沙丁鱼副产品油的组中，血清胆固醇和三酰甘油降低（分别降低8%和36%）。然而，血糖水平相似。HDL3-磷脂、HDL3-未酯化胆固醇和HDL3-载脂蛋白分别降低了56%、10%和12%。卵磷脂胆固醇酰基转移酶活性增加35%，HDL2-胆固醇酯含量升高12%。血清对氧磷酶-1活性增加25%。
在肥胖大鼠中，沙丁鱼副产品油可能通过改善胆固醇和三酰甘油的抗动脉粥样硬化代谢途径，对心血管风险具有保护作用。对氧磷酶-1活性的增加可保护脂蛋白免受氧化，从而特别增强了这种抗动脉粥样硬化作用。
