Nakamura K, Ishii A, Tamaki K
Department of Dermatology, Faculty of Medicine, Tokyo University Branch Hospital, University of Tokyo, Japan.
Arch Dermatol Res. 1990;282(4):253-7. doi: 10.1007/BF00371645.
Lectin-binding profiles of epidermal Langerhans cells (LCs) were investigated in three strains of mice using immunofluorescence procedures. Three lectin-binding profiles were observed in each strain of mice. Most epidermal LCs reacted with concanavalin A (Con A) and Ricinus communis agglutinin 1 (RCA-1), whereas none reacted with Dolichos biflorus agglutinin (DBA). Peanut agglutinin (PNA) and wheat germ agglutinin (WGA) reacted with some of the epidermal LCs. These binding profiles were similar from site to site of the body in all strains of mice. We also investigated the lectin-binding profiles of epidermal Ia positive (Ia+) cells migrating into the grafted skin up to 165 days after transplantation. BALB/c (H-2d) murine skin was grafted onto the back of (C3H/He x BALB/c)F1 (H-2k x H-2d) mice. The percentages of migrating I-A+ epidermal cells reactive with PNA and WGA were different from those of the normal epidermis soon after grafting and reached a normal level at 43 days after grafting. Our results demonstrated that there is a heterogeneous population of epidermal LCs defined by lectin-binding profiles.
利用免疫荧光法，对三种品系小鼠表皮朗格汉斯细胞（LCs）的凝集素结合谱进行了研究。在每个品系小鼠中均观察到三种凝集素结合谱。大多数表皮LCs与伴刀豆球蛋白A（Con A）和蓖麻凝集素1（RCA-1）反应，而无一与双花扁豆凝集素（DBA）反应。花生凝集素（PNA）和小麦胚凝集素（WGA）与部分表皮LCs反应。在所有品系小鼠中，这些结合谱在身体各部位均相似。我们还研究了移植后长达165天移入移植皮肤的表皮Ia阳性（Ia+）细胞的凝集素结合谱。将BALB/c（H-2d）小鼠皮肤移植到（C3H/He×BALB/c）F1（H-2k×H-2d）小鼠背部。移植后不久，与PNA和WGA反应的迁移I-A+表皮细胞百分比与正常表皮不同，并在移植后43天达到正常水平。我们的结果表明，存在由凝集素结合谱定义的异质性表皮LCs群体。
