Brysk M M, Snider J M
J Invest Dermatol. 1982 May;78(5):366-70. doi: 10.1111/1523-1747.ep12507473.
Epidermal cells were grown in a medium in which the Ca++ concentration controlled the stage of differentiation. Cell surface molecules of differentiated and undifferentiated cells were compared by lactoperoxidase-catalyzed iodination, by the interaction with 125I-lectins, and by the metabolic incorporation of L-(3H)-fucose. Molecular weights of the labeled components were determined by SDS-PAGE and autoradiography. After lactoperoxidase iodination, most of the radioactivity was found in polypeptide bands of 79,000, 65,000 and 56,000 daltons. The 79,000 band is the most intense for undifferentiated cells (and also for neoplastic cells) but disappears as differentiation proceeds. The 56,000 band is present in normal cells at all stages of differentiation but is absent from neoplastic cells. Glycoproteins reacted with 125I-lectins were found at 180,000, 130,000 and 85,000 daltons. The 130,000 band was the most prominent for differentiated cells labeled with wheat germ agglutinin but was essentially absent from the undifferentiated cells. With Ricinus communis agglutinin, this band was weaker for undifferentiated than for differentiated cells but was the most intense for both. After metabolic incorporation of tritiated fucose, radioactive glycoproteins were found at 130,000 and 85,000 daltons, with comparable intensities for differentiated and undifferentiated cells.
表皮细胞在一种培养基中生长，该培养基中钙离子浓度控制着分化阶段。通过乳过氧化物酶催化碘化、与125I-凝集素的相互作用以及L-(3H)-岩藻糖的代谢掺入，比较了分化细胞和未分化细胞的细胞表面分子。通过SDS-PAGE和放射自显影测定标记成分的分子量。乳过氧化物酶碘化后，大部分放射性出现在79,000、65,000和56,000道尔顿的多肽条带中。79,000道尔顿的条带在未分化细胞（以及肿瘤细胞）中最强烈，但随着分化的进行而消失。56,000道尔顿的条带在分化的所有阶段的正常细胞中都存在，但在肿瘤细胞中不存在。与125I-凝集素反应的糖蛋白在180,000、130,000和85,000道尔顿处被发现。130,000道尔顿的条带在用麦胚凝集素标记的分化细胞中最突出，但在未分化细胞中基本不存在。用蓖麻凝集素处理后，该条带在未分化细胞中比在分化细胞中弱，但在两者中都是最强烈的。氚化岩藻糖代谢掺入后，在130,000和85,000道尔顿处发现了放射性糖蛋白，分化细胞和未分化细胞的强度相当。
