来自嗜热栖热放线菌MB4的一种热稳定丙氨酸消旋酶的结晶及初步X射线研究。
Crystallization and preliminary X-ray study of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4.
作者信息
Dong Hui, Xu Shujing, Lu Xiaoyun, He Guangzheng, Zhao Ranran, Chen Shuai, Fu Sheng, Ju Jiansong
机构信息
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):660-2. doi: 10.1107/S1744309113011743. Epub 2013 May 25.
Abstract
Alanine racemase (Alr(MB4)), a dimeric PLP-dependent thermostable enzyme from the anaerobic eubacterium Thermoanaerobacter tengcongensis MB4, was expressed and purified with a His(6) tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K using a solution consisting of 0.1 M bis-tris pH 7.0, 22%(w/v) polyethylene glycol 4000. X-ray diffraction data were collected to 2.6 Å resolution. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with two protein molecules in an asymmetric unit.
摘要
丙氨酸消旋酶（Alr(MB4)）是一种来自嗜热栖热放线菌MB4的依赖磷酸吡哆醛的二聚体耐热酶，它以带有His(6)标签的形式进行表达和纯化，其形式适合进行X射线晶体学分析。晶体通过悬滴气相扩散法在289 K下生长，使用由0.1 M双三羟甲基氨基甲烷pH 7.0、22%（w/v）聚乙二醇4000组成的溶液。X射线衍射数据收集至2.6 Å分辨率。该晶体属于正交晶系空间群P2(1)2(1)2(1)，一个不对称单元中有两个蛋白质分子。
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