来自类芽孢杆菌OF4的碱性丙氨酸消旋酶的结晶及初步X射线研究。
Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4.
作者信息
Ju Jiansong, Qi Jianxun, Xu Shujing, Ohnishi Kouhei, Benedik Michael J, Xue Yanfen, Ma Yanhe
机构信息
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Feb 1;65(Pt 2):166-8. doi: 10.1107/S174430910900013X. Epub 2009 Jan 31.
Abstract
Alanine racemase (DadX(OF4)), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His(6) tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 291 K using a solution containing 1.4 M sodium/potassium phosphate pH 8.2. The protein crystallized in space group P2(1)2(1)2(1), with two protein molecules in the asymmetric unit.
摘要
丙氨酸消旋酶（DadX(OF4)）是一种来自嗜碱假芽孢杆菌OF4的二聚体内源性磷酸吡哆醛依赖性碱性酶，在大肠杆菌中表达，并通过His(6)标签纯化，得到适合X射线晶体学分析的形式。晶体通过悬滴气相扩散法在291 K下使用含有1.4 M磷酸钠/钾（pH 8.2）的溶液生长。该蛋白质在空间群P2(1)2(1)2(1)中结晶，不对称单位中有两个蛋白质分子。
相似文献
1
Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4.来自类芽孢杆菌OF4的碱性丙氨酸消旋酶的结晶及初步X射线研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Feb 1;65(Pt 2):166-8. doi: 10.1107/S174430910900013X. Epub 2009 Jan 31.
2
Characterization of endogenous pyridoxal 5'-phosphate-dependent alanine racemase from Bacillus pseudofirmus OF4.来自类芽孢杆菌OF4的内源性磷酸吡哆醛依赖性丙氨酸消旋酶的特性分析。
J Biosci Bioeng. 2009 Mar;107(3):225-9. doi: 10.1016/j.jbiosc.2008.11.005.
3
Crystallization and preliminary X-ray study of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4.来自嗜热栖热放线菌MB4的一种热稳定丙氨酸消旋酶的结晶及初步X射线研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):660-2. doi: 10.1107/S1744309113011743. Epub 2013 May 25.
4
Crystallization and preliminary X-ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4.来自类芽孢杆菌OF4的丙氨酸脱氢酶的结晶及初步X射线研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1227-30. doi: 10.1107/S1744309113025426. Epub 2013 Oct 17.
5
Crystallization and preliminary X-ray study of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1.铜绿假单胞菌PAO1生物合成丙氨酸消旋酶的结晶及初步X射线研究
Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1616-9. doi: 10.1107/S2053230X14022432. Epub 2014 Nov 14.
6
Structural features and kinetic characterization of alanine racemase from Bacillus pseudofirmus OF4.来自类芽孢杆菌OF4的丙氨酸消旋酶的结构特征和动力学表征
Biochem Biophys Res Commun. 2018 Feb 26;497(1):139-145. doi: 10.1016/j.bbrc.2018.02.041. Epub 2018 Feb 7.
7
Expression, crystallization and preliminary X-ray crystallographic analysis of alanine racemase from Acinetobacter baumannii OXA-23.鲍曼不动杆菌OXA-23丙氨酸消旋酶的表达、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):1041-4. doi: 10.1107/S1744309113022343. Epub 2013 Aug 21.
8
Crystallization and X-ray diffraction of an ectoine hydroxylase from Bacillus pseudofirmus OF4.来自类芽孢杆菌OF4的依克多因羟化酶的结晶及X射线衍射
Wei Sheng Wu Xue Bao. 2015 Nov 4;55(11):1468-74.
9
Crystallization and preliminary X-ray diffraction analysis of alanine racemase from Pseudomonas putida YZ-26.恶臭假单胞菌YZ-26丙氨酸消旋酶的结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Oct 1;68(Pt 10):1240-2. doi: 10.1107/S1744309112036974. Epub 2012 Sep 29.
10
Crystallization and preliminary crystallographic analysis of β-mannanase from Bacillus licheniformis.地衣芽孢杆菌β-甘露聚糖酶的结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Feb 1;67(Pt 2):217-20. doi: 10.1107/S1744309110049067. Epub 2011 Jan 22.
引用本文的文献
1
Crystallization and preliminary X-ray diffraction analysis of alanine racemase from Pseudomonas putida YZ-26.恶臭假单胞菌YZ-26丙氨酸消旋酶的结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Oct 1;68(Pt 10):1240-2. doi: 10.1107/S1744309112036974. Epub 2012 Sep 29.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Characterization of endogenous pyridoxal 5'-phosphate-dependent alanine racemase from Bacillus pseudofirmus OF4.来自类芽孢杆菌OF4的内源性磷酸吡哆醛依赖性丙氨酸消旋酶的特性分析。
J Biosci Bioeng. 2009 Mar;107(3):225-9. doi: 10.1016/j.jbiosc.2008.11.005.
3
Purification and preliminary crystallization of alanine racemase from Streptococcus pneumoniae.肺炎链球菌丙氨酸消旋酶的纯化及初步结晶
BMC Microbiol. 2007 May 17;7:40. doi: 10.1186/1471-2180-7-40.
4
A gene encoding alanine racemase is involved in spore germination in Bacillus thuringiensis.编码丙氨酸消旋酶的基因参与苏云金芽孢杆菌的孢子萌发。
Arch Microbiol. 2007 May;187(5):371-8. doi: 10.1007/s00203-006-0201-x. Epub 2006 Dec 13.
5
Alanine racemase of alfalfa seedlings (Medicago sativa L.): first evidence for the presence of an amino acid racemase in plants.苜蓿幼苗（紫花苜蓿）的丙氨酸消旋酶：植物中存在氨基酸消旋酶的首个证据。
Phytochemistry. 2006 May;67(9):856-60. doi: 10.1016/j.phytochem.2006.02.017. Epub 2006 Apr 17.
6
Purification, properties, and partial amino acid sequences of alanine racemase from the muscle of the black tiger prawn Penaeus monodon.黑虎虾（斑节对虾）肌肉中丙氨酸消旋酶的纯化、性质及部分氨基酸序列
Comp Biochem Physiol B Biochem Mol Biol. 2002 Nov;133(3):445-53. doi: 10.1016/s1096-4959(02)00187-2.
7
Functional characterization of alanine racemase from Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial alanine racemase.粟酒裂殖酵母丙氨酸消旋酶的功能特性：一种与细菌丙氨酸消旋酶相对应的真核生物酶。
J Bacteriol. 2001 Apr;183(7):2226-33. doi: 10.1128/JB.183.7.2226-2233.2001.
8
Reidentification of facultatively alkaliphilic Bacillus firmus OF4 as Bacillus pseudofirmus OF4.兼性嗜碱坚强芽孢杆菌OF4重新鉴定为类坚强芽孢杆菌OF4 。
Extremophiles. 2000 Feb;4(1):19-22. doi: 10.1007/s007920050003.
9
Tyrosine 265 of alanine racemase serves as a base abstracting alpha-hydrogen from L-alanine: the counterpart residue to lysine 39 specific to D-alanine.丙氨酸消旋酶的酪氨酸265作为从L-丙氨酸中提取α-氢的碱基：这是D-丙氨酸特有的赖氨酸39的对应残基。
J Biochem. 1999 Oct;126(4):781-6. doi: 10.1093/oxfordjournals.jbchem.a022517.
10
Solvent content of protein crystals.蛋白质晶体的溶剂含量。
J Mol Biol. 1968 Apr 28;33(2):491-7. doi: 10.1016/0022-2836(68)90205-2.
Zotero 插件