新型嗜冷卤代烷脱卤酶的晶体学分析：来自嗜冷嗜盐杆菌K5的DpcA和来自海杆菌属ELB17的DmxA。
Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17.
作者信息
Tratsiak Katsiaryna, Degtjarik Oksana, Drienovska Ivana, Chrast Lukas, Rezacova Pavlina, Kuty Michal, Chaloupkova Radka, Damborsky Jiri, Kuta Smatanova Ivana
机构信息
Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, CENAKVA and Institute of Complex Systems, Zamek 136, 373 33 Nove Hrady, Czech Republic.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):683-8. doi: 10.1107/S1744309113012979. Epub 2013 May 25.
Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 Å.
卤代烷脱卤酶是一种水解酶，具有广泛的潜在实际应用，如生物降解、生物传感、生物催化和细胞成像。新分离出的两种具有有趣催化特性的嗜冷卤代烷脱卤酶，即来自嗜冷嗜盐杆菌K5的DpcA和来自海杆菌属ELB17的DmxA，经过纯化后用于结晶实验。在优化结晶条件后，获得了具有衍射质量的晶体。收集了天然酶以及与选定配体（如1-溴己烷和1,2-二氯乙烷）形成的复合物的衍射数据集，分辨率范围为1.05至2.49 Å。
Zotero 插件