Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany.
Antonie Van Leeuwenhoek. 2013 Aug;104(2):207-16. doi: 10.1007/s10482-013-9939-8. Epub 2013 May 31.
A novel Gram-strain positive, aerobic, actinobacterial strain, designated CF11/1(T), was isolated from a sand sample obtained in the Sahara Desert, Chad. The black-pigmented isolate was aerobic and exhibited optimal growth from 25 to 35 °C at pH 6.0-8.0 and with 0-8 % (w/v) NaCl, indicating that it is a halotolerant mesophile. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The G+C content in the genome was 74.4 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a minor fraction of phosphatidylglycerol; MK-9(H4) was the dominant menaquinone, and galactose was detected as a diagnostic sugar. The major cellular fatty acid was branched-chain saturated acid iso-C16:0. Analysis of 16S rRNA gene sequences showed 95.3-98.6 % pairwise sequence identity with the members of the genus Geodermatophilus. Based on phenotypic and chemotaxonomic properties, as well as phylogenetic distinctiveness, the isolate represents a novel species, Geodermatophilus africanus, with the type strain CF11/1(T) (DSM 45422 = CCUG 62969 = MTCC 11556).
一株新型革兰氏阳性、需氧放线菌菌株，命名为 CF11/1(T)，从乍得撒哈拉沙漠的沙样中分离得到。该黑色色素产生菌为需氧菌，在 25-35°C、pH6.0-8.0 和 0-8%（w/v）NaCl 的条件下生长最佳，表明其是一种耐盐的嗜中温菌。该分离株的化学分类学和分子特征与地嗜皮菌属的成员描述相符。基因组中的 G+C 含量为 74.4 mol%。肽聚糖含有鉴定二氨基庚二酸的 meso-二氨基庚二酸。主要磷脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇和少量磷脂酰甘油；MK-9(H4)是主要的menaquinone，检测到半乳糖作为鉴定糖。主要细胞脂肪酸为支链饱和酸 iso-C16:0。16S rRNA 基因序列分析显示，与地嗜皮菌属的成员的序列相似度为 95.3-98.6%。基于表型和化学分类学特性以及系统发育上的独特性，该分离株代表了一个新的物种，非洲地嗜皮菌，其模式株为 CF11/1(T)（DSM 45422=CCUG 62969=MTCC 11556）。
