IFAPA - Instituto de Investigación y Formación Agraria y Pesquera, Centro Las Torres-Tomejil, Ctra. Sevilla-Cazalla de la Sierra, Km 12.2, C.P. 41200 Alcalá del Río, Sevilla, Spain.
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2013 Sep;63(Pt 9):3437-3443. doi: 10.1099/ijs.0.051201-0. Epub 2013 Mar 29.
A novel Gram-reaction-positive actinobacterial strain, designated CF5/3(T), was isolated from a sand sample obtained in the Sahara Desert, Chad. The greenish-black-pigmented isolate was aerobic and exhibited optimal growth from 25-40 °C at pH 6.0-10.0 with 0-1% (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G+C content of the genome of the novel strain was 75.5 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a minor fraction of phosphatidylglycerol. MK-9(H4) was the dominant menaquinone, and galactose was detected as a diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids: iso-C(15:0) and iso-C(16:0). Analysis of 16S rRNA gene sequences showed 95.6-98.8% pairwise sequence identity with the members of the genus Geodermatophilus. Based on phenotypic and chemotaxonomic properties, as well as phylogenetic distinctiveness, the isolate represents a novel species, Geodermatophilus normandii, with the type strain CF5/3(T) ( =DSM 45417(T) =CCUG 62814(T) =MTCC 11412(T)).
一株新型革兰氏阳性放线菌菌株，命名为 CF5/3(T)，从乍得撒哈拉沙漠的沙样中分离得到。该绿色到黑色色素的分离株为需氧菌，在 25-40°C、pH6.0-10.0、0-1%（w/v）NaCl 的条件下生长最佳。分离株的化学分类和分子特征与地衣芽孢杆菌属的成员描述相符。新型菌株的基因组 DNA G+C 含量为 75.5mol%。肽聚糖含有中二氨基庚二酸作为诊断性二氨基酸。主要磷脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇和少量磷脂酰甘油。MK-9(H4) 是主要的menaquinone，检测到半乳糖作为诊断性糖。主要细胞脂肪酸为支链饱和脂肪酸：异-C(15:0)和异-C(16:0)。16S rRNA 基因序列分析显示与地衣芽孢杆菌属成员的序列相似度为 95.6-98.8%。基于表型和化学分类特征以及系统发育的独特性，该分离株代表了一个新的物种，即 Normandii 地衣芽孢杆菌，其模式菌株为 CF5/3(T)（ =DSM 45417(T) =CCUG 62814(T) =MTCC 11412(T)）。
