Suh Yeoun Suk, Kamruzzaman Mohammad, Alam Al-Mahmnur, Lee Sang Hak, Kim Young Ho, Kim Gyu-Man, Dang Trung Dung
Department of Chemistry, Kyungpook National University, Daegu, 702-701, South Korea.
Luminescence. 2014 May;29(3):248-53. doi: 10.1002/bio.2536. Epub 2013 May 30.
A sensitive determination of a synthetic fluoroquinolone antibacterial agent, moxifloxacin (MOX), by an enhanced chemiluminescence (CL) method using a microfluidic chip is described. The microfluidic chip was fabricated by a soft-lithographic procedure using polydimethyl siloxane (PDMS). The fabricated PDMS microfluidic chip had three-inlet microchannels for introducing the sample, chemiluminescent reagent and oxidant, and a 500 µm wide, 250 µm deep and 82 mm long microchannel. An enhanced CL system, luminol-ferricyanide, was adopted to analyze the MOX concentration in a sample solution. CL light was emitted continuously after mixing luminol and ferricyanide in the presence of MOX on the PDMS microfluidic chip. The amount of MOX in the luminol-ferricyanide system influenced the intensity of the CL light. The linear range of MOX concentration was 0.14-55.0 ng/mL with a correlation coefficient of 0.9992. The limit of detection (LOD) and limit of quantification (LOQ) were 0.06 and 0.2 ng/mL respectively. The presented method afforded good reproducibility, with a relative standard deviation (RSD) of 1.05% for 10 ng/mL of MOX, and has been successfully applied for the determination of MOX in pharmaceutical and biological samples.
描述了一种使用微流控芯片通过增强化学发光（CL）法灵敏测定合成氟喹诺酮抗菌剂莫西沙星（MOX）的方法。该微流控芯片采用聚二甲基硅氧烷（PDMS）通过软光刻工艺制造。所制造的PDMS微流控芯片具有用于引入样品、化学发光试剂和氧化剂的三入口微通道，以及一个宽500 µm、深250 µm、长82 mm的微通道。采用增强型CL系统鲁米诺-铁氰化物来分析样品溶液中的MOX浓度。在PDMS微流控芯片上，在MOX存在的情况下，将鲁米诺和铁氰化物混合后会持续发出CL光。鲁米诺-铁氰化物系统中MOX的量会影响CL光的强度。MOX浓度的线性范围为0.14 - 55.0 ng/mL，相关系数为0.9992。检测限（LOD）和定量限（LOQ）分别为0.06和0.2 ng/mL。所提出的方法具有良好的重现性，对于10 ng/mL的MOX，相对标准偏差（RSD）为1.05%，并且已成功应用于药物和生物样品中MOX的测定。
