D'Antò Vincenzo, Raucci Maria Grazia, Guarino Vincenzo, Martina Stefano, Valletta Rosa, Ambrosio Luigi
Institute of Composite and Biomedical Materials (IMCB), National Research Council of Italy (CNR), Naples, Italy.
Department of Neurosciences, Reproductive Sciences and Oral Sciences, University of Naples 'Federico II', Italy.
J Tissue Eng Regen Med. 2016 Feb;10(2):E147-54. doi: 10.1002/term.1768. Epub 2013 May 31.
Our goal was to characterize the response of human mesenchymal stem cells (hMSCs) to a novel composite scaffold for bone tissue engineering. The hydroxyapatite-polycaprolactone (HA-PCL) composite scaffolds were prepared by a sol-gel method at room temperature and the scaffold morphology was investigated by scanning electron microscopy (SEM)/energy-dispersive spectroscopy (EDS) to validate the synthesis process. The response of two different lines of hMSCs, bone-marrow-derived human mesenchymal stem cells (BMSCs) and dental pulp stem cells (DPSCs) in terms of cell proliferation and differentiation into the osteoblastic phenotype, was evaluated using Alamar blue assay, SEM, histology and alkaline phosphatase activity. Our results indicate that tissue engineering by means of composite HA-PCL scaffolds may represent a new therapeutic strategy to repair craniofacial bone defects.
我们的目标是表征人间充质干细胞（hMSCs）对用于骨组织工程的新型复合支架的反应。通过室温溶胶 - 凝胶法制备羟基磷灰石 - 聚己内酯（HA - PCL）复合支架，并通过扫描电子显微镜（SEM）/能量色散光谱（EDS）研究支架形态以验证合成过程。使用Alamar蓝测定法、SEM、组织学和碱性磷酸酶活性评估了两种不同系的hMSCs，即骨髓来源的人间充质干细胞（BMSCs）和牙髓干细胞（DPSCs）在细胞增殖和成骨细胞表型分化方面的反应。我们的结果表明，通过复合HA - PCL支架进行组织工程可能代表一种修复颅面骨缺损的新治疗策略。
