Schmid Jakob, Schwarz Sascha, Fischer Martina, Sudhop Stefanie, Clausen-Schaumann Hauke, Schieker Matthias, Huber Robert
Center for Applied Tissue Engineering and Regenerative Medicine (CANTER), University of Applied Sciences Munich, Munich, Germany.
Laboratory of Experimental Surgery and Regenerative Medicine (ExperiMed), Ludwig-Maximilians University Munich, Munich, Germany.
J Tissue Eng. 2019 Jul 23;10:2041731419859157. doi: 10.1177/2041731419859157. eCollection 2019 Jan-Dec.
A manufacturing process for sheet-based stacked scaffolds (SSCs) based on laser-cutting (LC) was developed. The sheets consist of Polycaprolactone/Hydroxyapatite (PCL/HA) composite material. Single sheets were cut from a PCL/HA foil and stacked to scaffolds with interconnecting pores of defined sizes. HA quantities up to 50% were processable with high reproducibility, while the accuracy was dependent on the applied laser power. The smallest achievable pore sizes were about 40 µm, while the smallest stable solid structures were about 125 µm. The human mesenchymal stem cell line SCP-1 was cultured on the manufactured PCL/HA scaffolds. The cells developed a natural morphology and were able to differentiate to functional osteoblasts. The generation of PCL/HA SSCs via LC offers new possibilities for tissue engineering (TE) approaches. It is reliable and fast, with high resolution. The SSC approach allows for facile cell seeding and analysis of cell fate within the three-dimensional cell culture, thus allowing for the generation of functional tissue constructs.
开发了一种基于激光切割（LC）的片状堆叠支架（SSC）制造工艺。这些薄片由聚己内酯/羟基磷灰石（PCL/HA）复合材料组成。从PCL/HA箔上切割出单张薄片，并堆叠成具有特定尺寸互连孔隙的支架。高达50%的HA含量可实现高重现性加工，而精度取决于所施加的激光功率。可实现的最小孔径约为40 µm，而最小的稳定固体结构约为125 µm。人骨髓间充质干细胞系SCP-1在制造的PCL/HA支架上培养。细胞呈现出自然形态，并能够分化为功能性成骨细胞。通过LC生成PCL/HA SSC为组织工程（TE）方法提供了新的可能性。它可靠、快速且具有高分辨率。SSC方法允许在三维细胞培养中轻松进行细胞接种和细胞命运分析，从而实现功能性组织构建体的生成。
