Xu Xiu-Fang, Xin Yi, Zhang Ying, Huang Yi-Min, Gu Yun, Li Na, Li Wen-Bin, Zhou Yu-Jie, Zhang Zhao-Guang
Department of Molecular Biology, Capital Medical University Affiliated with Beijing Anzhen Hospital, Beijing Institute of Heart, Lung & Blood Vessel Diseases Beijing 100029, China.
Int J Clin Exp Med. 2013 May 22;6(5):404-12. Print 2013.
The immunosuppressive agent cyclosporin A has been proven to reduce the rejection rate and prolong the survival time of transplanted hearts. But some reports showed that cyclosporine A did not completely suppress the rejection. We performed in vitro studies to model a time course to observe the effect of cyclosporin A.
The experiment was divided into a control group (group I), an antigen group (group II), a cyclosporin A group (group III) and an antigen + cyclosporin A group (group IV). After transplantation, at 2 h, 6 h, 12 h, 24 h, 48 h and 72 h, leukocyte molecules were monitored.
The expression of IL-2R peaked at 12 h in group II and at 6 h in group III. There was a gradual decline in the expression of the P59 gene in group I, positive expression at 2 h and between 12 h and 24 h in group II, in group IV, there was a decrease at 48 h. The expression of the CD4 gene was lowest at 2 h in group I and at 6 h in group II. CD4 expression then quickly increased to a maximum at 48 h in group III, at 2 h in group IV. There was a minimal expression was reached at 12 h in group I and IV and at 6 h in group III in the expression of the CD8 gene.
Alloantigen induced lymphocytes to release IL-2R and P59 and stimulated the induction of the CD4 gene' transcription for 6 h. Cyclosporin A stimulated the release of IL-2R for 2 h. These results provide an in vitro basis for describing the time phases of rejection inhibited by cyclosporin A.
免疫抑制剂环孢素A已被证明可降低移植心脏的排斥率并延长其存活时间。但一些报告显示，环孢素A并未完全抑制排斥反应。我们进行了体外研究以模拟时间进程，观察环孢素A的作用。
实验分为对照组（I组）、抗原组（II组）、环孢素A组（III组）和抗原+环孢素A组（IV组）。移植后，在2小时、6小时、12小时、24小时、48小时和72小时监测白细胞分子。
II组中IL-2R的表达在12小时达到峰值，III组在6小时达到峰值。I组中P59基因的表达逐渐下降，II组在2小时以及12小时至24小时呈阳性表达，IV组在48小时表达下降。I组中CD4基因的表达在2小时最低，II组在6小时最低。然后，III组中CD4表达在48小时迅速增加至最大值，IV组在2小时达到最大值。I组和IV组中CD8基因的表达在12小时达到最低，III组在6小时达到最低。
同种异体抗原诱导淋巴细胞释放IL-2R和P59，并刺激CD4基因转录诱导6小时。环孢素A刺激IL-2R释放2小时。这些结果为描述环孢素A抑制排斥反应的时间阶段提供了体外依据。
