Sato T
Department of Anatomy and Cytobiology, Justus Liebig-University, Giessen, Federal Republic of Germany.
Arch Histol Cytol. 1990 May;53(2):141-6. doi: 10.1679/aohc.53.141.
Using the histochemical method for the demonstration of NADPH-diaphorase activity, the pineal organ of the frog and rat was investigated in serial sections. A positive NADPH-diaphorase activity was demonstrated in pinealocytes and nerve cells in the pineal organ of the frog, but not in the rat. An intense activity existed in the apical portion of the photosensitive pinealocytes of the frog. Large NADPH-diaphorase positive nerve cells (15-20 microns in diameter) were located within the parenchyma of the pineal organ in the frog. Large NADPH-positive nerve cells were more numerous in the rostral than in the caudal portion of the organ, but the intensely stained cells, counting 25-35 in number, showed almost equal distribution and number in the ventral and the dorsal aspect of the pineal organ. In their staining ability, NADPH-diaphorase positive pineal nerve cells resembled retinal amacrine cells. The results in the pineal organ of the frog are discussed in light of previous morphological findings using the acetylcholinesterase reaction, and with electrophysiological results.
运用组织化学方法来显示NADPH - 黄递酶活性，对青蛙和大鼠的松果体器官进行了连续切片研究。在青蛙松果体器官的松果体细胞和神经细胞中显示出阳性NADPH - 黄递酶活性，但在大鼠中未显示。在青蛙感光松果体细胞的顶端部分存在强烈活性。大型NADPH - 黄递酶阳性神经细胞（直径15 - 20微米）位于青蛙松果体器官的实质内。大型NADPH阳性神经细胞在器官的吻侧比尾侧更多，但染色强烈的细胞，数量为25 - 35个，在松果体器官的腹侧和背侧显示出几乎相等的分布和数量。在染色能力方面，NADPH - 黄递酶阳性松果体神经细胞类似于视网膜无长突细胞。根据先前使用乙酰胆碱酯酶反应的形态学发现以及电生理结果，对青蛙松果体器官的结果进行了讨论。
