Department of Biotechnology, Ming Chuan University, Gui-Shan, Taiwan.
J Appl Microbiol. 2013 Sep;115(3):673-8. doi: 10.1111/jam.12265. Epub 2013 Jun 24.
Purification and characterization of a novel bacteriocin produced by strain Enterococcus faecium D081821.
Enterococcus faecium D081821, isolated from the traditional Taiwanese fermented food dochi (fermented black beans), was previously found to produce a bacteriocin against Listeria monocytogenes and some Gram-positive bacteria. This bacteriocin, termed enterocin TW21, was purified from culture supernatant by ammonium sulfate precipitation, Sep-Pak C18 cartridge, ion-exchange and gel filtration chromatography. Mass spectrometry analysis showed the mass of the peptide to be approximately 5300·6 Da. The N-terminal amino acid sequencing yielded a partial sequence NH2 -ATYYGNGVYxNTQK by Edman degradation, and it contains the consensus class IIa bacteriocin motif YGNGV in the N-terminal region. The open reading frame (ORF) encoding the bacteriocin was identified from the draft genome sequence of Enterococcus faecium D081821, and sequence analysis of this peptide indicated that enterocin TW21 is a novel bacteriocin.
Enterococcus faecium D081821 produced a bacteriocin named enterocin TW21, the molecular weight and amino acid sequence both revealed it to be a novel bacteriocin.
A new member of class IIa bacteriocin was identified. This bacteriocin shows great inhibitory ability against L. monocytogenes and could be applied as a natural food preservative.
从屎肠球菌 D081821 中分离纯化并鉴定一种新型细菌素。
屎肠球菌 D081821 从传统台湾发酵食品豆齿（发酵黑豆）中分离出来，先前被发现能够产生一种针对李斯特菌和一些革兰氏阳性菌的细菌素。这种细菌素被命名为肠球菌 TW21，通过硫酸铵沉淀、Sep-Pak C18 小柱、离子交换和凝胶过滤色谱从培养上清液中纯化得到。质谱分析显示该肽的分子量约为 5300.6 Da。通过 Edman 降解得到 N 端氨基酸序列的部分序列 NH2 -ATYYGNGVYxNTQK，并在 N 端区域含有公认的 IIa 类细菌素模体 YGNGV。从屎肠球菌 D081821 的草图基因组序列中鉴定出编码该细菌素的开放阅读框（ORF），并对该肽的序列分析表明肠球菌 TW21 是一种新型细菌素。
屎肠球菌 D081821 产生了一种名为肠球菌 TW21 的细菌素，其分子量和氨基酸序列均表明它是一种新型细菌素。
鉴定出一种新型 IIa 类细菌素。这种细菌素对李斯特菌具有很强的抑制能力，可作为天然食品防腐剂。
