Gurocak Simay, Karabulut Ercan, Karadag Nese, Ozgor Dincer, Ozkeles Neslihan, Karabulut Aysun Bay
Department of Radiation Oncology, Inonu University School of Medicine, Malatya, Turkey.
Asian Pac J Cancer Prev. 2013;14(4):2367-70. doi: 10.7314/apjcp.2013.14.4.2367.
In recent years, due to modern lifestyles and exposure to chemical carcinogens, cancer cases are steadily increasing. From this standpoint, azoxymethane (AOM), a chemical carcinogen which causes de novo liver damage, and resveratrol, which is an antioxidant found in foods and protects against oxidative stress damage, are of interest. We here aimed to evaluate whether resveratrol could protect the liver tissues from the effects of AOM.
The study was conducted in 4 groups, each consisting of seven rats, the first receiving only AOM (2 times per week, 5 mg/kg), group 2 AOM and resveratrol (2 times a week, 20 mg/kg), group 3 assessed only as a control and group 4 administered only resveratrol. At the end of the seventh week, the rats were sacrificed. Rat liver MDA, NO, GSH levels were analyzed biochemically, as well as the tissues being evaluated histopathologically.
MDA and NO increased in AOM group as signs of increased oxidative stress. The group concomitantly administered resveratrol was been found to be significantly decreased in MDA and NO levels and increased in GSH activity. However, there were no significant findings on histopathological evaluation.
In the light of these results, resveratrol appears to exert protective effect on oxidative stress in the liver tissue due to deleterious effects of chemical carcinogens.
近年来，由于现代生活方式以及接触化学致癌物，癌症病例在稳步增加。从这个角度来看，能引发肝脏原发性损伤的化学致癌物偶氮甲烷（AOM）以及食物中发现的具有抗氧化作用、可防止氧化应激损伤的白藜芦醇受到关注。我们在此旨在评估白藜芦醇是否能保护肝脏组织免受AOM的影响。
该研究在4组中进行，每组由7只大鼠组成，第一组仅接受AOM（每周2次，5毫克/千克），第2组接受AOM和白藜芦醇（每周2次，20毫克/千克），第3组仅作为对照组进行评估，第4组仅给予白藜芦醇。在第7周结束时，处死大鼠。对大鼠肝脏的丙二醛（MDA）、一氧化氮（NO）、谷胱甘肽（GSH）水平进行生化分析，并对组织进行组织病理学评估。
作为氧化应激增加的迹象，AOM组的MDA和NO升高。发现同时给予白藜芦醇的组中，MDA和NO水平显著降低，GSH活性升高。然而，组织病理学评估未发现显著结果。
根据这些结果，白藜芦醇似乎对化学致癌物的有害作用所导致的肝脏组织氧化应激具有保护作用。
