Kurus M, Bay Karabulut A, Taslidere E, Otlu O
Department of Histology and Embryology, Izmir Katip Celebi Universitesi, Izmir, Turkey.
Department of Biochemistry, Inonu Universitesi Tip fakultesi, Malatya, Turkey.
Andrologia. 2017 Aug;49(6). doi: 10.1111/and.12674. Epub 2016 Aug 3.
To evaluate the protective effects of Resveratrol (RES) on azoxymethane (AOM)-induced testicular damage using histopathology and biochemical analyses, 28 male rats were randomly divided into four groups. Groups were control, RES, AOM and ARES. At the end of the 7 weeks, following routine tissue processing procedure, testis sections were stained with haematoxylin-eosin and Masson's trichrome. The blood samples were taken for biochemical analysis of testosterone, total oxidative stress, total antioxidant status and oxidative stress index. Degenerative changes in the seminiferous tubules such as atrophy, loss in the number of germ cells and arrested spermatogenic cell, and increase in the connective tissue of the tunica albuginea in the groups with AOM treatment were found. RES treatment (ARES) reduced the number of affected seminiferous tubules significantly (p < .05) compared to AOM alone. The testosterone levels in AOM group were significantly lower than in the control group (p < .05). The total oxidative stress levels were significantly higher in AOM group compared to control group (p < .05). The total antioxidant status levels in ARES group were significantly higher than in the AOM group (p < .05). This study results suggest that an antioxidant like Resveratrol may be useful for decreasing the harmful effects of azoxymethane.
为了通过组织病理学和生化分析评估白藜芦醇（RES）对乙基亚硝基脲（AOM）诱导的睾丸损伤的保护作用，将28只雄性大鼠随机分为四组。分组为对照组、RES组、AOM组和ARES组。在7周结束时，按照常规组织处理程序，将睾丸切片用苏木精-伊红和马松三色染色法染色。采集血样用于睾酮、总氧化应激、总抗氧化状态和氧化应激指数的生化分析。发现在接受AOM处理的组中，生精小管出现退行性变化，如萎缩、生殖细胞数量减少和生精细胞停滞，以及白膜结缔组织增加。与单独使用AOM相比，RES处理（ARES）显著减少了受影响的生精小管数量（p < 0.05）。AOM组的睾酮水平显著低于对照组（p < 0.05）。与对照组相比，AOM组的总氧化应激水平显著更高（p < 0.05）。ARES组的总抗氧化状态水平显著高于AOM组（p < 0.05）。本研究结果表明，像白藜芦醇这样的抗氧化剂可能有助于降低乙基亚硝基脲的有害影响。
