Luo Ting, Chen Long, He Ping, Hu Qian-Cheng, Zhong Xiao-Rong, Sun Yu, Yang Yuan-Fu, Tian Ting-Lun, Zheng Hong
Department of Head and Neck and Mammary Oncology, Cancer Center and State Key Laboratory of Biotherapy, Laboratory of Molecular Diagnosis of Cancer, West China Hospital, Sichuan University, Chengdu, China.
Asian Pac J Cancer Prev. 2013;14(4):2433-7. doi: 10.7314/apjcp.2013.14.4.2433.
Vascular endothelial growth factor (VEGF) is a potent regulator of angiogenesis and thereby involved in the development and progression of solid tumours. Associations between three VEGF gene polymorphisms (-634 G/C, +936 C/T, and +1612 G/A) and breast cancer risk have been extensively studied, but the currently available results are inconclusive. Our aim was to investigate associations between three VEGF gene polymorphisms and breast cancer risk in Chinese Han patients. We performed a hospital-based case-control study including 680 female incident breast cancer patients and 680 female age-matched healthy control subjects. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was performed to detect the three VEGF gene polymorphisms. We observed that women carriers of +936 TT genotypes [odds ratio (OR) =0.46, 95% confidence interval (CI) = 0.28, 0.76; P=0.002] or 936 T-allele (OR=0.81, 95% CI= 0.68, 0.98; P=0.03) had a protective effect concerning the disease. Our study suggested that the +1612G/A polymorphism was unlikely to be associated with breast cancer risk. The -634CC genotype was significantly associated with high tumor aggressiveness [large tumor size (OR=2.63, 95% CI=1.15, 6.02; P=0.02) and high histologic grade (OR=1.47, 95% CI= 1.06, 2.03; P=0.02)]. The genotypes were not related with other tumor characteristics such as regional or distant metastasis, stage at diagnosis, or estrogen or progesterone receptor status. Our study revealed that the VEGF -634 G/C and +936 C/T gene polymorphisms may be associated with breast cancer in Chinese Han patients.
血管内皮生长因子（VEGF）是血管生成的强效调节因子，因此参与实体瘤的发生和发展。VEGF基因的三种多态性（-634G/C、+936C/T和+1612G/A）与乳腺癌风险之间的关联已得到广泛研究，但目前可得的结果尚无定论。我们的目的是调查中国汉族患者中VEGF基因的三种多态性与乳腺癌风险之间的关联。我们进行了一项基于医院的病例对照研究，纳入680例女性乳腺癌初发患者和680例年龄匹配的女性健康对照者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析检测三种VEGF基因多态性。我们观察到，携带+936TT基因型的女性[比值比（OR）=0.46，95%置信区间（CI）=0.28，0.76；P=0.002]或936T等位基因（OR=0.81，95%CI=0.68，0.98；P=0.03）对该疾病具有保护作用。我们的研究表明，+1612G/A多态性不太可能与乳腺癌风险相关。-634CC基因型与高肿瘤侵袭性显著相关[肿瘤体积大（OR=2.63，95%CI=1.15，6.02；P=0.02）和高组织学分级（OR=1.47，95%CI=1.06，2.03；P=0.02）]。这些基因型与其他肿瘤特征无关，如区域或远处转移、诊断时的分期或雌激素或孕激素受体状态。我们的研究表明，VEGF -634G/C和+936C/T基因多态性可能与中国汉族患者的乳腺癌有关。
