Division of Head, Neck and Breast Surgery, Department of Surgery, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Mol Med Rep. 2013 Oct;8(4):1242-50. doi: 10.3892/mmr.2013.1607. Epub 2013 Jul 31.
Polymorphisms in the promoter and 5' untranslated region of vascular endothelial growth factor (VEGF) have been associated with VEGF levels. To investigate the role of VEGF polymorphisms in breast cancer, the VEGF ‑2578C/A, ‑1498C/T, ‑1154G/A and ‑634G/C polymorphisms were genotyped in 483 breast cancer patients and 524 healthy controls. VEGF mRNA levels in breast cancer tissue were determined using semi‑quantitative RT‑PCR. The genotypes, ‑634G/C and ‑634C/C, were associated with an increased risk for breast cancer when compared with the ‑634G/G genotype. The VEGF ‑634G/C genotype was associated with tumor size >20 mm, perineural invasion and stage II‑IV. Individuals with ‑634C/C had lower disease‑free survival. Patients with the VEGF ‑634C/C genotype exhibited the highest VEGF mRNA levels. High VEGF mRNA expression correlated with tumor size >20 mm, presence of lymphovascular invasion and axillary nodal metastasis. These observations suggested that VEGF ‑634G/C polymorphisms have a significant role in breast cancer susceptibility and aggressiveness.
血管内皮生长因子 (VEGF) 启动子和 5' 非翻译区的多态性与 VEGF 水平相关。为了研究 VEGF 多态性在乳腺癌中的作用，对 483 例乳腺癌患者和 524 例健康对照者的 VEGF-2578C/A、-1498C/T、-1154G/A 和 -634G/C 多态性进行了基因分型。采用半定量 RT-PCR 法测定乳腺癌组织中 VEGF mRNA 水平。与 -634G/G 基因型相比，-634G/C 和 -634C/C 基因型与乳腺癌发病风险增加相关。VEGF-634G/C 基因型与肿瘤大小>20mm、神经周围浸润和 II-IV 期相关。-634C/C 基因型的个体无病生存率较低。VEGF-634C/C 基因型患者的 VEGF mRNA 水平最高。高 VEGF mRNA 表达与肿瘤大小>20mm、存在血管淋巴管浸润和腋窝淋巴结转移相关。这些观察结果表明，VEGF-634G/C 多态性在乳腺癌易感性和侵袭性方面具有重要作用。
