Nano-organic Photoelectronic Laboratory and Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
ACS Appl Mater Interfaces. 2013 Jun 26;5(12):5811-4. doi: 10.1021/am401391g. Epub 2013 Jun 17.
Very recently, one-dimensional nanowire (NW) sensors have attracted great attention as smart optical endoscopes to probe and manipulate intracellular biological processes. However, NWs often have limited optical response to the intracellular environment changes. In this work, a near-infrared nanowire optical endoscope for high-resolution intracellular pH detection was developed by integrating the advantages of silicon nanowires and surface-enhanced Raman spectroscopy (SERS). This optical endoscope has a high-resolution, sensitive response to local pH changes over the wide range of pH 4.0-9.0, an important range for most biological processes in cells, with high reproducibility, good reversibility, and at least one-week stability in an aqueous environment. The results indicate the great potential of a single SiNW SERS endoscope for intracellular pH monitoring.
最近，一维纳米线（NW）传感器作为智能光学内窥镜，在探测和操纵细胞内生物过程方面引起了极大的关注。然而，NW 通常对细胞内环境变化的光学响应有限。在这项工作中，通过整合硅纳米线和表面增强拉曼光谱（SERS）的优势，开发了一种近红外纳米线光学内窥镜，用于高分辨率的细胞内 pH 检测。这种光学内窥镜对局部 pH 值变化具有高分辨率、灵敏的响应，在 pH 4.0-9.0 的宽范围内，这是细胞中大多数生物过程的重要范围，具有高重现性、良好的可逆性，在水相环境中至少稳定一周。结果表明，单个 SiNW SERS 内窥镜在细胞内 pH 监测方面具有巨大的潜力。
