Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Ecotoxicol Environ Saf. 2013 Aug;94:1-7. doi: 10.1016/j.ecoenv.2013.04.005. Epub 2013 May 30.
The effects of Cu-tolerant bacteria strain USTB-O on Cu accumulation, plant growth and reduction of Cu toxicity in wheat seedlings Triticum aestivum L. were investigated. The strain was identified as belonging to Bacillus species and showed a specific tolerance to Cu through binding the Cu ions to the cell walls to reduce their entry into the cells. The bacteria not only increased Cu accumulation in wheat seedlings, but also secreted indole-3-acetic acid (IAA) and therefore promoted plant growth. Moreover, the bacteria effectively improved the antioxidant defence system to alleviate the oxidative damage induced by Cu. The bacteria promoted superoxide dismutase (SOD) in both shoots and roots to reduce superoxide radicals. The bacteria stimulated all enzymes activities under Cu exposure conditions, peroxidase (POD) and catalase (CAT) in shoots and ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) in roots were major enzymes to eliminate H2O2 in wheat seedlings.
研究了耐铜细菌菌株 USTB-O 对小麦幼苗（Triticum aestivum L.）铜积累、植物生长和减轻铜毒性的影响。该菌株被鉴定为芽孢杆菌属，并通过将铜离子结合到细胞壁上来减少铜进入细胞，从而表现出对铜的特异性耐受。该细菌不仅增加了小麦幼苗中的铜积累，还分泌了吲哚-3-乙酸（IAA），从而促进了植物生长。此外，细菌还有效地改善了抗氧化防御系统，以减轻铜引起的氧化损伤。细菌促进了超氧化物歧化酶（SOD）在地上部和根部的活性，以减少超氧自由基。细菌在铜暴露条件下刺激了所有酶的活性，地上部的过氧化物酶（POD）和过氧化氢酶（CAT）以及根部的抗坏血酸过氧化物酶（APX）和脱氢抗坏血酸还原酶（DHAR）是消除小麦幼苗中 H2O2 的主要酶。
