Northeast Dairy Foods Research Center, Department of Food Science, Cornell University, Ithaca, NY 14853, USA.
J Dairy Sci. 2013 Aug;96(8):4870-84. doi: 10.3168/jds.2012-6542. Epub 2013 May 30.
Our objective was to develop an alternative process to produce low-fat Cheddar cheese (LFCC) by combining reduced-fat Cheddar cheese (RFCC) made by a fat-removal process with micellar casein concentrate (MCC) to try to achieve the texture and flavor characteristics of full-fat Cheddar cheese (FFCC). The production of LFCC was replicated 3 times. The MCC was produced by ultrafiltration of skim milk, followed by 3 stages of microfiltration, and the final MCC was spray dried. The LFCC was formulated to achieve 6% fat, 28% protein, and 1.2% salt by a combination of RFCC, MCC powder, salt, and water. The 6% fat target was selected to comply with the FDA standard for a low-fat label claim. The pH of the LFCC mixture was adjusted to 5.3 by lactic acid. Rennet was added to the LFCC mixture, followed by pressing and packaging. Chemical and sensory data were analyzed by ANOVA using the Proc GLM of SAS to determine if any differences in chemical composition and sensory properties were present among different cheeses. Descriptive sensory scores were used to construct a principal component analysis biplot to visualize flavor profile differences among cheeses. The LFCC had 83% less fat, 32% less sodium, and higher protein and moisture content than FFCC. When the cheese texture was evaluated in the context of a filled-gel model consisting of matrix and filler (100% minus percentage of matrix) the LFCC had lower filler volume than FFCC, yet the LFCC had a softer texture than FFCC. The LFCC contained some of the original FFCC cheese matrix that had been disrupted by the fat-removal process, and this original FFCC matrix was embedded in the new LFCC matrix formed by the action of rennet on casein from the continuous phase of hydrated MCC. Thus, the texture of the LFCC was desirable and was softer than the FFCC it was made from, whereas commercial RFCC (50 and 75% fat reduction) were firmer than the FFCC. The sulfur flavor in LFCC was closer to FFCC than commercial RFCC. The LFCC had bitter and grape-tortilla off-flavors that came from the dried MCC ingredient. The commercial RFCC and LFCC made in this study were missing the typical aged Cheddar character (catty, nutty, fruity, brothy, milk fat flavors) found in aged FFCC. Future work to improve the flavor of LFCC made by the process described in this study should include the addition of a flavoring ingredient (e.g., enzyme-modified cheese) to enhance the aged Cheddar flavors and mask undesirable flavors.
我们的目标是通过将脱脂切达干酪(RFCC)与胶束酪蛋白浓缩物(MCC)结合,开发一种替代方法来生产低脂切达干酪(LFCC),以尝试达到全脂切达干酪(FFCC)的质地和风味特征。LFCC 的生产重复了 3 次。MCC 通过对脱脂牛奶进行超滤,然后进行 3 级微滤,最后进行喷雾干燥而制成。LFCC 的配方是通过 RFCC、MCC 粉末、盐和水的组合来实现 6%的脂肪、28%的蛋白质和 1.2%的盐。选择 6%的脂肪目标是为了符合 FDA 对低脂标签声明的标准。通过添加乳酸将 LFCC 混合物的 pH 调节至 5.3。向 LFCC 混合物中添加凝乳酶,然后进行压榨和包装。使用 SAS 的 Proc GLM 对化学和感官数据进行方差分析,以确定不同奶酪之间在化学成分和感官特性上是否存在差异。使用描述性感官评分构建主成分分析双标图,以可视化奶酪之间的风味差异。LFCC 的脂肪含量比 FFCC 低 83%,钠含量低 32%,蛋白质和水分含量更高。当在由基质和填充剂(基质的 100%减去百分比)组成的填充凝胶模型中评估奶酪质地时,LFCC 的填充剂体积低于 FFCC,但 LFCC 的质地比 FFCC 更柔软。LFCC 含有一些通过脱脂过程破坏的原始 FFCC 奶酪基质,这些原始 FFCC 基质嵌入在由 MCC 连续相的水合作用形成的新 LFCC 基质中,由凝乳酶作用于酪蛋白形成。因此,LFCC 的质地是理想的,比其制成的 FFCC 更柔软,而商业 RFCC(脂肪减少 50%和 75%)则比 FFCC 更硬。LFCC 的硫磺味更接近 FFCC,而不是商业 RFCC。LFCC 有苦味和葡萄玉米饼的异味,来自干燥的 MCC 成分。本研究中制备的商业 RFCC 和 LFCC 缺少在陈年 FFCC 中发现的典型陈年切达干酪特征(猫味、坚果味、水果味、肉汤味、乳脂味)。为了改善本研究中所述方法制备的 LFCC 的风味,未来的工作应包括添加调味成分(例如,酶改性奶酪)以增强陈年切达干酪的风味并掩盖不良风味。