Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Thorvaldsensvej 57, DK-1871 Frederiksberg C, Denmark.
Vet Parasitol. 2013 Oct 18;197(1-2):360-3. doi: 10.1016/j.vetpar.2013.05.004. Epub 2013 May 14.
In order to further characterize the immune response around the viable or degenerating Taenia solium cysts in the pig brain, the involvement of cysteine protease in the immune evasion was assessed. Brain tissues from 30 adult pigs naturally infected with T. solium cysticercosis were subjected to histopathology using hematoxylin and eosin stain, and immunohistochemistry using caspase-3 antibodies. Histopathological evaluation revealed lesions of stage I which was characterized by presence of viable parasite surrounded with minimal to moderate inflammatory cells and stage III characterized by the presence of a disintegrating parasite surrounded with high inflammatory cells. The results of immunohistochemistry indicated caspase-3 positive cells interspaced between inflammatory infiltrate mainly in stage I lesions, indicating the presence of cysteine protease. This result confirms the earlier hypothesis that cysteine protease may play a role in inducing immune evasion through apoptosis around viable T. solium cysts.
为了进一步描述猪脑中活的或退化的猪带绦虫囊虫周围的免疫反应，评估了半胱氨酸蛋白酶在免疫逃避中的作用。使用苏木精和伊红染色对 30 头自然感染猪带绦虫囊尾蚴的成年猪的脑组织进行组织病理学检查，并使用 caspase-3 抗体进行免疫组织化学检查。组织病理学评估显示病变处于 I 期，其特征是活寄生虫周围有少量至中度炎症细胞，III 期特征是分解的寄生虫周围有大量炎症细胞。免疫组织化学结果表明 caspase-3 阳性细胞散布在炎症浸润物之间，主要存在于 I 期病变中，表明存在半胱氨酸蛋白酶。这一结果证实了早期的假设，即半胱氨酸蛋白酶可能通过活的猪带绦虫囊虫周围的细胞凋亡来发挥诱导免疫逃避的作用。
