Center for Medical Physics and Technology, Biophysics Group, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany.
Biochem Biophys Res Commun. 2013 Jun 28;436(2):246-51. doi: 10.1016/j.bbrc.2013.05.084. Epub 2013 May 30.
Mechanical forces play an important role in the organization, growth, maturation, and function of living tissues. At the cellular level, the transmission of forces from outside the cell through cell-matrix and cell-cell contacts are believed to control spreading, motility, maturation as well as intracellular signaling cascades that may change many characteristics in cells. We looked at cell populations of mouse embryonic fibroblasts that are deficient of focal adhesion kinase (FAK) and examined their mechanical profile. We observed that the lack of FAK induces a mesenchymal-epithelial switch including the regulation of adherens junctions via E-cadherin, leading to increased cell-cell-cohesion. Our results show that the absence of FAK influences the macroscopic cell colony spreading in two (2D) and three (3D) dimensions as well as the velocity fields of the tissue, the single cell persistence and correlation length, changing from an independent to a collective mode of migration. Additionally, the single cell size in the sheet decreases significantly.
机械力在组织、生长、成熟和活体组织功能中起着重要作用。在细胞水平上，细胞外的力通过细胞基质和细胞-细胞接触传递，被认为可以控制细胞的扩展、运动、成熟以及可能改变细胞许多特性的细胞内信号级联。我们观察了缺乏粘着斑激酶 (FAK) 的小鼠胚胎成纤维细胞群体，并检查了它们的力学特征。我们发现，缺乏 FAK 会诱导上皮-间充质转化，包括通过 E-钙粘蛋白调节粘着连接，从而增加细胞间的黏附。我们的结果表明，FAK 的缺失会影响二维 (2D) 和三维 (3D) 宏观细胞集落的扩展，以及组织的速度场、单个细胞的持久性和相关长度，从独立模式向集体模式迁移。此外，薄片中的单个细胞尺寸显著减小。
