Matsuo S, Ichikawa H, Wakisaka S, Akai M
First Department of Oral Anatomy, Osaka University, Japan.
Cell Tissue Res. 1990 May;260(3):521-8. doi: 10.1007/BF00297232.
The influence of colchicine on the addition of 3H-galactose to the enamel protein in secretory ameloblasts of cultured germs of rat molar tooth was investigated by light- and electron-microscopic radioautography. In tooth germs cultured without colchicine, the reaction products of 3H-galactose were observed over Golgi cisternae at early chase times and then localized over the enamel with time. In tooth germs cultured with colchicine, the silver grains were seen over the Golgi cisternae, condensing granules and accumulated secretory granules. Some grains also appeared with time over the pale granular material precipitated in the intercellular space with colchicine treatment. In quantitative analysis with light microscopic radioautography, values of silver grain counts over the unit area (100 microns2) on ameloblasts and enamel of colchicine-treated tooth germs were significantly lower at both 0 min and 30 min chase after 30 min pulse than those of control tooth germs, respectively. This finding indicates that colchicine diminished the incorporation of 3H-galactose into the secretory ameloblast of cultured tooth germs. It is suggested that colchicine decreases the activity of the Golgi apparatus with regard to the addition of sugar to the synthesizing glycoprotein in the secretory ameloblast.
通过光学显微镜和电子显微镜放射自显影术，研究了秋水仙碱对大鼠磨牙牙胚培养物中分泌期成釉细胞釉蛋白添加3H-半乳糖的影响。在未用秋水仙碱培养的牙胚中，在早期追踪时间，3H-半乳糖的反应产物在高尔基体潴泡上被观察到，然后随着时间推移定位于釉质上。在用秋水仙碱培养的牙胚中，银颗粒出现在高尔基体潴泡、浓缩颗粒和积累的分泌颗粒上。随着时间的推移，在用秋水仙碱处理的细胞间空间中沉淀的淡颗粒物质上也出现了一些颗粒。在光学显微镜放射自显影的定量分析中，在30分钟脉冲后0分钟和30分钟追踪时，秋水仙碱处理的牙胚的成釉细胞和釉质上单位面积（100平方微米）的银颗粒计数分别显著低于对照牙胚。这一发现表明秋水仙碱减少了3H-半乳糖掺入培养牙胚的分泌期成釉细胞中。提示秋水仙碱在分泌期成釉细胞中糖添加到合成糖蛋白方面降低了高尔基体的活性。
