Matsuo S, Inai T, Kurisu K, Kiyomiya K, Kurebe M
Department of Toxicology, School of Veterinary Medicine, University of Osaka Prefecture, Japan.
Arch Toxicol. 1996;70(7):420-9. doi: 10.1007/s002040050294.
Fluoride, which is an environmental toxicant, is a potent inducer of mottled enamel in humans and rats. To define the influence of fluoride on the secretory pathway in enamel fluorosis, mottled enamel was induced in the incisor tooth germs of rats by subcutaneous injections of sodium fluoride for 4 days, and then morphological and cytochemical changes of the secretory ameloblast were examined in the tooth germs with HRP-labeled lectin (Con A, GS-I, SBA and PNA) and En3 antibody labeling amelogenins. The accumulation of small vesicles on the route of the secretory pathway between the rER and the Golgi apparatus, disorder of Golgi stacks, and formation of abnormal large granules in distal cytoplasm were seen in the secretory ameloblast. Lectin staining patterns of the secretory ameloblast indicated the disturbance of the vesicular transport between the rER and the Golgi apparatus, and disorganization of the Golgi stack. Immunolabeling of the cell showed disruption of the sorting and fusion process on the secretory pathway. These results suggest that the fluoride disturbs the intracellular transport in the synthesis-secretory pathway of the ameloblast, and that this effect of fluoride on the synthesis-secretory pathway participates in the formation of enamel fluorosis.
氟化物是一种环境毒物，是人类和大鼠牙釉质斑纹的强效诱导剂。为了确定氟化物对氟斑牙分泌途径的影响，通过皮下注射氟化钠4天在大鼠切牙牙胚中诱导形成斑纹牙釉质，然后用HRP标记的凝集素（伴刀豆球蛋白A、GS-I、大豆凝集素和花生凝集素）和抗釉原蛋白的En3抗体标记，检查牙胚中分泌性成釉细胞的形态和细胞化学变化。在分泌性成釉细胞中可见小泡在粗面内质网和高尔基体之间的分泌途径上积累、高尔基体堆叠紊乱以及远端细胞质中形成异常大颗粒。分泌性成釉细胞的凝集素染色模式表明粗面内质网和高尔基体之间的小泡运输受到干扰，高尔基体堆叠紊乱。细胞的免疫标记显示分泌途径上的分选和融合过程受到破坏。这些结果表明，氟化物扰乱了成釉细胞合成-分泌途径中的细胞内运输，并且氟化物对合成-分泌途径的这种作用参与了氟斑牙的形成。
