[麻疹被动血凝试验的研制及其在血清学诊断中的应用]
[Development of passive hemagglutination assay of measles and utilization in serological diagnosis].
作者信息
Zhang R
机构信息
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 1990 Apr;11(2):103-7.
The passive hemagglutination assay (PHA) was developed. Preparation of antigen and its stability, sensitivity and specificity was studied. The result showed that the yield of ultrasonic treated antigen is high and its titer has no change in 4 degrees C storage at least 1 month. The sensitized sheep erythrocyte with this antigen have a high specificity and sensitivity. The lysophilized blood cells could stored for a long time in 4 degrees C. A comparison of PHA with hemagglutination inhibition test was carried out by examination of 354 samples of sera from healthy children. The GMT of titer of antibodies of measles were 26.20 and 13.92 respectively. The result showed that the two methods were highly related.
开发了被动血凝试验（PHA）。对抗原的制备及其稳定性、敏感性和特异性进行了研究。结果表明，经超声处理的抗原产量高，其效价在4℃保存至少1个月无变化。用该抗原致敏的绵羊红细胞具有高特异性和敏感性。冻干血细胞可在4℃长期保存。通过检测354份健康儿童血清样本，对PHA与血凝抑制试验进行了比较。麻疹抗体效价的几何平均滴度分别为26.20和13.92。结果表明这两种方法高度相关。
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