Karadzhnov I, Ignatov G, Khristova V
Vet Med Nauki. 1979;16(8):65-71.
The preparation and standardization of the ingredients for PHA is described in detail. Formalin treated erythrocites in a 10% concentration treated with 1:20 000 tannin were used. The antigen concentrated by ultracentrifugation properly sensibilizes the erythrocites and their titer does not change in case of its dilution up to 1:8. The non-concentrated virus antigen has a low covering potential only if it is either not diluted, or in a 1:2 dilution and sensibilizes the erythrocites to a lesser extent. The great advantage of the use of concentrated antigen is that it equalizes antigens obtained from various lots. The optimal time for loading by erythrocites is 1 h, and a longer sensibilization has no influence on the effect of loading. It was proven that the sensibilization of formalin treated erythrocites allows long storage of the produced antigen. The tannin treated formalinized erythrocites can be stored up to 20 days without any change in their ability to be antigen covered, while the sensibilized erythrocites are not changed for 6 days and up to the 30th day their titer is twice lower, but this is of no practical importance.
详细描述了PHA成分的制备和标准化。使用经1:20000单宁处理的10%浓度的福尔马林处理红细胞。通过超速离心浓缩的抗原能适当地使红细胞致敏，并且在稀释至1:8的情况下其滴度不变。未浓缩的病毒抗原只有在未稀释或1:2稀释时才具有低覆盖潜力，并且使红细胞致敏的程度较小。使用浓缩抗原的一大优势在于它能使来自不同批次的抗原达到均衡。红细胞加载的最佳时间为1小时，更长时间的致敏对加载效果没有影响。已证明福尔马林处理的红细胞致敏可使所产生的抗原长期保存。单宁处理的福尔马林化红细胞可保存20天，其被抗原覆盖的能力无任何变化，而致敏红细胞在6天内无变化，直至第30天其滴度降低一半，但这在实际中并无重要意义。
