miRNA 前体基因变异与中国人群结直肠癌风险的相关性研究。
Association between genetic variants in pre-miRNA and colorectal cancer risk in a Chinese population.
机构信息
Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, People's Republic of China.
出版信息
J Cancer Res Clin Oncol. 2013 Aug;139(8):1405-10. doi: 10.1007/s00432-013-1456-7. Epub 2013 Jun 2.
INTRODUCTION
Single-nucleotide polymorphisms (SNPs) in pre-miRNAs may alter microRNA expression levels or processing and then contribute to the susceptibility of cancer development. We hypothesized that SNPs in pre-miRNAs may be associated with the risk of colorectal cancer (CRC).
MATERIALS AND METHODS
We genotyped four common polymorphisms (i.e., rs11614913, rs3746444, rs2910164, and rs2292832) in pre-miRNAs of 353 CRC patients and 540 healthy controls to investigate the association between the SNPs and the risk of CRC using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
RESULTS
The rs11614913 CT, TT genotypes, and T allele were associated with an increased risk of CRC compared with the CC genotype and C allele (CT vs. CC: OR = 7.34, 95% CI 3.76-14.34; TT vs. CC: OR = 13.66, 95% CI 6.76-27.6; T vs. C: OR = 1.99, 95% CI 1.63-2.42, respectively). Interestingly, using the rs2910164 GG genotype as a reference, the rs2910164 GC genotype was associated with an increased risk of CRC (OR = 1.49, 95% CI 1.02-2.18), whereas the rs2910164 CC genotype was associated with a decreased risk of CRC (OR = 0.58, 95% CI 0.37-0.93). When compared with the rs2910164G allele, rs2910164 C allele was associated with a reduced risk of CRC (OR = 0.80, 95% CI 0.66-0.97, p = 0.02).
CONCLUSION
These findings suggest that rs11614913 and rs2910164 polymorphisms may be associated with the etiology of CRC.
介绍
miRNA 前体中的单核苷酸多态性（SNPs）可能改变 miRNA 的表达水平或加工方式，从而导致癌症易感性的改变。我们假设 miRNA 前体中的 SNPs 可能与结直肠癌（CRC）的发病风险相关。
材料和方法
我们对 353 例 CRC 患者和 540 例健康对照者的 miRNA 前体中的 4 个常见 SNPs（rs11614913、rs3746444、rs2910164 和 rs2292832）进行基因分型，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测方法，探讨 SNPs 与 CRC 发病风险之间的关系。
结果
与 CC 基因型和 C 等位基因相比，rs11614913 CT、TT 基因型和 T 等位基因与 CRC 的发病风险增加相关（CT 与 CC：OR=7.34，95%CI 3.76-14.34；TT 与 CC：OR=13.66，95%CI 6.76-27.6；T 与 C：OR=1.99，95%CI 1.63-2.42）。有趣的是，以 rs2910164 GG 基因型为参照，rs2910164 GC 基因型与 CRC 的发病风险增加相关（OR=1.49，95%CI 1.02-2.18），而 rs2910164 CC 基因型与 CRC 的发病风险降低相关（OR=0.58，95%CI 0.37-0.93）。与 rs2910164G 等位基因相比，rs2910164C 等位基因与 CRC 的发病风险降低相关（OR=0.80，95%CI 0.66-0.97，p=0.02）。
结论
这些发现提示 rs11614913 和 rs2910164 多态性可能与 CRC 的发病机制有关。
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