Ke Tao-Wei, Wu Ming-Hsien, Chen Ying-Jing, Yueh Te-Cheng, Shih Hou-Yu, Chang Yu-Chen, Wang Yun-Chi, Tsai Chia-Wen, Bau DA-Tian, Chang Wen-Shin
Department of Colorectal Surgery, China Medical University Hospital, Taichung, Taiwan, R.O.C.
Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
In Vivo. 2025 Jul-Aug;39(4):1891-1901. doi: 10.21873/invivo.13988.
BACKGROUND/AIM: Matrix metalloproteinase-11 (MMP-11) is a member of the MMP enzyme family, known for its critical function in extracellular matrix turnover and tissue restructuring. This study aimed to investigate the potential associations between four single-nucleotide polymorphisms (SNPs)-rs738791, rs2267029, rs738792, and rs28382575-and colorectal cancer (CRC) susceptibility in a Taiwanese cohort.
A total of 362 CRC patients and non-cancer controls were genotyped using the restriction fragment length polymorphism method. The distribution of genotypes and alleles was assessed, and conformity to Hardy-Weinberg equilibrium was confirmed for all examined loci (>0.05).
No statistically significant differences were observed in the genotype frequencies of rs738791, rs2267029, rs738792, or rs28382575 between CRC patients and healthy controls ( for trend=0.8640, 0.6638, 0.3275, and 0.8051, respectively). Allelic analyses further revealed lack of associations with CRC risk regarding rs738791 T allele (OR=1.06, 95%CI=0.85-1.32, =0.6550), rs2267029 A allele (OR=1.11, 95%CI=0.88-1.40, =0.4073), rs738792 C allele (OR=1.02, 95%CI=0.81-1.29, =0.9055), and rs28382575 C allele (OR=1.17, 95% CI=0.67-2.04, =0.6718). Interestingly, individuals carrying the CT or TT genotypes of rs738791 were more likely to present with larger tumor sizes (≥5 cm, =0.0298) and absence of metastasis (=0.0218). Moreover, the AG or AA genotypes of rs2267029 were significantly associated with advanced clinical stages (III-IV, =0.0007).
Although the investigated polymorphisms were not associated with CRC susceptibility, the rs738791 and rs2267029 variant genotypes may serve as potential prognostic biomarkers.
背景/目的：基质金属蛋白酶-11（MMP-11）是MMP酶家族的成员，以其在细胞外基质周转和组织重构中的关键作用而闻名。本研究旨在调查台湾队列中四个单核苷酸多态性（SNP）——rs738791、rs2267029、rs738792和rs28382575——与结直肠癌（CRC）易感性之间的潜在关联。
使用限制性片段长度多态性方法对362例CRC患者和非癌症对照进行基因分型。评估基因型和等位基因的分布，并确认所有检测位点均符合哈迪-温伯格平衡（>0.05）。
CRC患者与健康对照之间，rs738791、rs2267029、rs738792或rs28382575的基因型频率未观察到统计学显著差异（趋势检验P值分别为0.8640、0.6638、0.3275和0.8051）。等位基因分析进一步显示，rs738791的T等位基因（OR=1.06，95%CI=0.85-1.32，P=0.6550）、rs2267029的A等位基因（OR=1.11，95%CI=0.88-1.40，P=0.4073）、rs738792的C等位基因（OR=1.02，95%CI=0.8I-1.29，P=0.9055）和rs28382575的C等位基因（OR=1.17，95%CI=0.67-2.04，P=0.6718）与CRC风险均无关联。有趣的是，携带rs738791的CT或TT基因型的个体更有可能出现较大的肿瘤尺寸（≥5 cm，P=0.0298）且无转移（P=0.0218）。此外，rs2267029的AG或AA基因型与晚期临床分期（III-IV期，P=0.0007）显著相关。
虽然所研究的多态性与CRC易感性无关，但rs738791和rs2267029的变异基因型可能作为潜在的预后生物标志物。
