Williams Mark A
ISMB Biophysics Centre, Institute of Structural and Molecular Biology, Birkbeck, University of London, London, UK.
Methods Mol Biol. 2013;1008:3-34. doi: 10.1007/978-1-62703-398-5_1.
Here are described the basic mechanisms governing the interactions between proteins and their natural or manmade ligands, together with the principles underlying their analysis. The consequences of these principles are detailed for the simplest case of one-to-one binding. The general features of experimental measurements of biomolecular interactions arise from properties of the molecules involved and, thus, are common to many methods of detection. Consequently, an understanding of these principles greatly simplifies adoption and comparison of experimental methods and provides the rationale underlying many common protocols. In seeking to understand and interpret the results of experiments or identify possible sources of error these fundamental ideas are a constant guide.
本文描述了蛋白质与其天然或人工配体之间相互作用的基本机制，以及对其进行分析的基本原理。针对最简单的一对一结合情况，详细阐述了这些原理的结果。生物分子相互作用实验测量的一般特征源于所涉及分子的性质，因此，许多检测方法都具有这些特征。因此，理解这些原理极大地简化了实验方法的采用和比较，并为许多常见方案提供了理论依据。在试图理解和解释实验结果或识别可能的误差来源时，这些基本思想是始终如一的指导。
