Lowe Peter N, Vaughan Cara K, Daviter Tina
Biomolecular Interactions Consultancy, Hertford, UK.
Methods Mol Biol. 2013;1008:63-99. doi: 10.1007/978-1-62703-398-5_3.
Experimental approaches to detect, measure, and quantify protein-ligand binding, along with their theoretical bases, are described. A range of methods for detection of protein-ligand interactions is summarized. Specific protocols are provided for a nonequilibrium procedure pull-down assay, for an equilibrium direct binding method and its modification into a competition-based measurement and for steady-state measurements based on the effects of ligands on enzyme catalysis.
本文描述了用于检测、测量和定量蛋白质-配体结合的实验方法及其理论基础。总结了一系列检测蛋白质-配体相互作用的方法。提供了非平衡下拉分析、平衡直接结合方法及其基于竞争的测量方法的改进以及基于配体对酶催化作用的稳态测量的具体方案。
