Daviter Tina, Fronzes Rémi
ISMB Biophysics Centre, Institute of Structural and Molecular Biology, Birkbeck, University of London, London, UK.
Methods Mol Biol. 2013;1008:35-62. doi: 10.1007/978-1-62703-398-5_2.
Most biophysical experiments require protein samples of high quality and accurately determined concentration. This chapter attempts to compile basic information on the most common methods to assess the purity, dispersity, and stability of protein samples. It also reminds of methods to measure protein concentration and of their limits. The idea is to make aware and remind of the range of methods available and of commonly overlooked pitfalls. The aim is to enable experimenters to fully characterize their preparations of soluble or membrane proteins and gain reliable and reproducible results from their experimental work.
大多数生物物理实验都需要高质量且浓度精确测定的蛋白质样品。本章试图汇编有关评估蛋白质样品纯度、分散性和稳定性的最常用方法的基本信息。它还提及了测量蛋白质浓度的方法及其局限性。目的是让人们了解并提醒大家注意可用方法的范围以及常见的被忽视的陷阱。目标是使实验人员能够全面表征其可溶性或膜蛋白制剂，并从实验工作中获得可靠且可重复的结果。
