Martin-Luther- Universität Halle-Wittenberg, Institut f u r Biotechnologie, Kurt-Mothes-Strasse 3, D-06120 Halle Saale, Germany.
Biol Chem. 2013 Aug;394(8):1091-6. doi: 10.1515/hsz-2013-0136.
Drosophila Toll receptors are involved in embryonic development and in the immune response of adult flies. In both processes, the Toll receptor ligand is the NGF-like cystine knot protein Spätzle. Here we present the expression of Toll receptor ectodomain in Schneider cells at high yields and demonstrate a high affinity interaction with the refolded and trypsin-processed Spätzle cystine knot domain dimer. Poorly and anisotropically diffracting crystals of the complex could be improved by deglycosylation and dehydration, paving the way for structural analyses of the Toll-Spätzle interaction.
果蝇 Toll 受体参与胚胎发育和成年果蝇的免疫反应。在这两个过程中，Toll 受体配体是神经生长因子样胱氨酸结蛋白 Spätzle。在这里，我们以高产量表达 Schneider 细胞中的 Toll 受体胞外结构域，并证明其与重折叠和胰蛋白酶处理的 Spätzle 胱氨酸结结构域二聚体具有高亲和力相互作用。通过去糖基化和脱水处理，可以改善复合物的较差和各向异性衍射晶体，为 Toll-Spätzle 相互作用的结构分析铺平道路。
