用于芯片上磷酸肽富集的聚合物和聚合物微流控器件上TiO纳米颗粒薄膜的火焰气溶胶沉积法
Flame Aerosol Deposition of TiO Nanoparticle Films on Polymers and Polymeric Microfluidic Devices for On-Chip Phosphopeptide Enrichment.
作者信息
Rudin Thomas, Tsougeni Katerina, Gogolides Evangelos, Pratsinis Sotiris E
机构信息
Particle Technology Laboratory, Institute of Process Engineering, Department of Mechanical and Process Engineering, ETH Zurich 8092 Zurich, Switzerland.
出版信息
Microelectron Eng. 2012 Sep;97:341-344. doi: 10.1016/j.mee.2012.04.016.
Direct and fast (10s of seconds) deposition of flame-made, high surface-area aerosol films on polymers and polymeric microfluidic devices is demonstrated. Uniform TiO nanoparticle films were deposited on cooled Poly(methyl methacrylate) (PMMA) substrates by combustion of titanium(IV) isopropoxide (TTIP) - xylene solution sprays. Films were mechanically stabilized by in-situ annealing with a xylene spray flame. Plasma-etched microfluidic chromatography columns, comprising parallel microchannels were also coated with such nanoparticle films without any microchannel deformation. These microcolumns were successfully used in metal-oxide affinity chromatography (MOAC) to selectively trap phosphopeptides on these high surface-area nanostructured films. The chips had a high capacity retaining 1.2 μg of standard phosphopeptide. A new extremely fast method is developed for MOAC microchip stationary phase fabrication with applications in proteomics.
本文展示了一种直接且快速（数十秒）的方法，可将火焰制成的高表面积气溶胶薄膜沉积在聚合物和聚合物微流控装置上。通过燃烧异丙醇钛（TTIP）-二甲苯溶液喷雾，在冷却的聚甲基丙烯酸甲酯（PMMA）基板上沉积了均匀的TiO纳米颗粒薄膜。通过用二甲苯喷雾火焰进行原位退火，使薄膜得到机械稳定。包含平行微通道的等离子体蚀刻微流控色谱柱也涂覆了这种纳米颗粒薄膜，且没有任何微通道变形。这些微柱成功用于金属氧化物亲和色谱（MOAC），以在这些高表面积纳米结构薄膜上选择性捕获磷酸肽。这些芯片具有高容量，可保留1.2μg标准磷酸肽。开发了一种全新的极快速方法，用于制造MOAC微芯片固定相，并应用于蛋白质组学。
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