a Dipartimento di Scienze Farmaceutiche , Facoltà di Farmacia , via Bonanno 6, 56100 , Pisa , Italy.
Nat Prod Res. 2013;27(23):2212-5. doi: 10.1080/14786419.2013.800977. Epub 2013 Jun 3.
Several procedures of extraction with solvents for the simultaneous determination of vitamin C and some vitamins belonging to the B group (thiamine, riboflavine, nicotinic acid and nicotinamide) in multivitamin preparations and in artichokes (Cynara cardunculus subsp. scolymus [L.] Hegi) were developed. Different experimental conditions were used, in terms of heat treatment, composition and pH of the extraction mixture, with particular attention to high-temperature steps; purification of the extracts with solid phase extraction and stabilisation through lyophilisation were discussed. Analyses of the extracts were conducted by capillary electrophoresis in micellar electrokinetic chromatography modality. Borate buffer at pH 8.2 was used, and sodium dodecyl sulphate was added to the background electrolyte as surfactant. A range of linearity was determined and calibration curves were plotted for all the analytes.
开发了几种溶剂提取方法，用于同时测定多种维生素制剂和朝鲜蓟（Cynara cardunculus subsp. scolymus [L.] Hegi）中的维生素 C 和一些 B 族维生素（硫胺素、核黄素、烟酸和烟酰胺）。实验条件不同，包括热处理、提取混合物的组成和 pH 值，特别注意高温步骤；讨论了通过固相萃取对提取物进行纯化和通过冷冻干燥进行稳定化。通过胶束电动色谱模式的毛细管电泳对提取物进行分析。使用 pH 值为 8.2 的硼酸盐缓冲液，并在背景电解质中添加十二烷基硫酸钠作为表面活性剂。确定了一系列线性范围，并为所有分析物绘制了校准曲线。
