miRNAs 基因多态性与埃及女性类风湿关节炎的关联。
Association of microRNAs genes polymorphisms with rheumatoid arthritis in Egyptian female patients.
机构信息
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
出版信息
Joint Bone Spine. 2013 Dec;80(6):626-31. doi: 10.1016/j.jbspin.2013.03.005. Epub 2013 Jun 2.
OBJECTIVE
To investigate whether miRNA-499 (rs3746444) and miRNA-146a (rs2910164) genes polymorphisms are independent factors for rheumatoid arthritis (RA) in Egyptians, and whether they influence disease severity and activity.
METHODS
Two hundred and seventeen RA patients and 245 healthy controls were enrolled in this study. Polymorphisms of miRNA-146a and miRNA-499 genes were detected using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).
RESULTS
The miRNA-499 CT genotype was an independent factor of RA. The miRNA-499 CT, CC genotypes and C allele frequencies were significantly increased in erosive RA group. Moreover, the heterozygote CT had more severe and more active form of the disease compared with homozygote CC or TT. However, we did not find any significant association of miRNA-146a polymorphism with RA risk, severity, and activity.
CONCLUSION
The miRNA-499 polymorphism is an independent factor of RA, and influences disease severity and activity.
目的
探讨 miRNA-499(rs3746444)和 miRNA-146a(rs2910164)基因多态性是否为埃及人类风湿关节炎(RA)的独立因素，以及它们是否影响疾病严重程度和活动度。
方法
本研究纳入了 217 例 RA 患者和 245 例健康对照者。采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)检测 miRNA-146a 和 miRNA-499 基因的多态性。
结果
miRNA-499 CT 基因型是 RA 的一个独立因素。侵蚀性 RA 组的 miRNA-499 CT、CC 基因型和 C 等位基因频率显著增加。此外，杂合子 CT 与纯合子 CC 或 TT 相比，疾病的严重程度和活动度更高。然而，我们没有发现 miRNA-146a 多态性与 RA 风险、严重程度和活动度之间存在任何显著关联。
结论
miRNA-499 多态性是 RA 的一个独立因素，并且影响疾病的严重程度和活动度。
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